CLUSTAL W (1.83) multiple sequence alignment TBK1_Hsap MQ-------------------------------------------STSNHLWLLSDILGQ IKKe_Hsap MQ-------------------------------------------STANYLWHTDDLLGQ IKKb_Hsap MSWSP-------------------------------------SLTTQTCGAWEMKERLGT IKKa_Hsap MERPP-------------------------------------GLRPGAGGPWEMRERLGT ird5_Dmel MITVVFCFSDCHGGIQILGRMSSVN-K----------IKLNENNKMHSFGNWERCRNLGE ik2_Dmel MSF----------------------------------------LRGSVSYVWCTTSVLGK IKKe_Mmus MQ-------------------------------------------STTNYLWHTDDLLGQ IKKb_Mmus MSWSP-------------------------------------SLPTQTCGAWEMKERLGT IKKa_Mmus MERPP-------------------------------------GLRPGAGGPWEMRERLGT TBK1_Mmus MQ-------------------------------------------STSNHLWLLSDILGQ TBK1_Spur MAATQG-------------------------------------IRSTKTYIWYLKDVLGT IKKe_Spur MSDKDH-I------------QGWSRNFVEVMEQNGRARQPVPNIRSTPGYIIFLDTPLGQ IKKa_Spur MP-------------------------------------------SPSGDQWQFKKLLGS AqueK157_Aque MSIRK---------------------------------------------NWR-RAPIGR AqueK158_Aque MAYNYQQV------------RPAFIGGG------------DETEYYSLSYIWSSKDKLGQ * :* TBK1_Hsap GATANVFRGR-------------------------------------------------- IKKe_Hsap GATASVYKAR-------------------------------------------------- IKKb_Hsap GGFGNVIRWH-------------------------------------------------- IKKa_Hsap GGFGNVCLYQ-------------------------------------------------- ird5_Dmel GGFGLVIHWR-------------------------------------------------- ik2_Dmel GATGSVFQGV-------------------------------------------------- IKKe_Mmus GATASVYKAR-------------------------------------------------- IKKb_Mmus GGFGNVIRWH-------------------------------------------------- IKKa_Mmus GGFGNVSLYQ-------------------------------------------------- TBK1_Mmus GATANVFRGR-------------------------------------------------- TBK1_Spur GATSSVYLGR-------------------------------------------------- IKKe_Spur GATSKVYGGR-------------------------------------------------- IKKa_Spur GSFGEVLLYQLGVGRKVGRFEAESVDWSRCMWSRFGVGEESVRPCCGVWLASLWCLVGVV AqueK157_Aque GSFGDVSLFT-------------------------------------------------- AqueK158_Aque GATGQVFAGY-------------------------------------------------- *. . * TBK1_Hsap ------------------------------------------------------------ IKKe_Hsap ------------------------------------------------------------ IKKb_Hsap ------------------------------------------------------------ IKKa_Hsap ------------------------------------------------------------ ird5_Dmel ------------------------------------------------------------ ik2_Dmel ------------------------------------------------------------ IKKe_Mmus ------------------------------------------------------------ IKKb_Mmus ------------------------------------------------------------ IKKa_Mmus ------------------------------------------------------------ TBK1_Mmus ------------------------------------------------------------ TBK1_Spur ------------------------------------------------------------ IKKe_Spur ------------------------------------------------------------ IKKa_Spur VVSGWRRCGVGLVSMSSQFGVDANVGLASMWCHFGLVCGVDEESVCPRCGVGLVLMWSQF AqueK157_Aque ------------------------------------------------------------ AqueK158_Aque ------------------------------------------------------------ TBK1_Hsap ----------HKKTGDLFAIKVFNNISFLRPVD---------VQMREFEVLKKL-NHKNI IKKe_Hsap ----------NKKSGELVAVKVFNTTSYLRPRE---------VQVREFEVLRKL-NHQNI IKKb_Hsap ----------NQETGEQIAIKQCRQELSPRNRE---------RWCLEIQIMRRL-THPNV IKKa_Hsap ----------HRELDLKIAIKSCRLELSTKNRE---------RWCHEIQIMKKL-NHANV ird5_Dmel ----------NRTTGREIATKHIKEMGALS-ADQQVK--LSERWNKELNWSRQFKNFPHI ik2_Dmel ----------NKITGESVAVKTFNPYSHMRPAD---------VQMREFEALKKV-NHENI IKKe_Mmus ----------NKKSGEVVAVKVFNSASYRRPPE---------VQVREFEVLRRL-NHQNI IKKb_Mmus ----------NQATGEQIAIKQCRQELSPKNRD---------RWCLEIQIMRRL-NHPNV IKKa_Mmus ----------HRELDLKIAIKSCRLELSSKNRE---------RWCHEIQIMKKL-DHANV TBK1_Mmus ----------HKKTGDLYAVKVFNNISFLRPVD---------VQMREFEVLKKL-NHKNI TBK1_Spur ----------HKKNGDVVAVKMFNHLSYMRPRD---------VQQREFEVLLKL-NHKNI IKKe_Spur ----------HKKLGKRVACKVPHTANLFRMGT---------DMERESQVLLRL-RNQNI IKKa_Spur GVDVVSVRRQCGETSEYIALKRCKSILQLE---------NLKRWRDEVEFMRNL-EHPNI AqueK157_Aque -----------SNDGEQIALKEMKLE-PLRNLDSHERDKMIVRLRNEIKIMWEH-NHPNL AqueK158_Aque ----------SKRSGDKVAIKMFTSPSEYKAMQ---------SEMQTIKALK---THPNI . * * : :: TBK1_Hsap VKLFAIEEE---------TTTRHKVLIMEFCPCGSLYT-VLEEPSNAYGLPESEFLIVLR IKKe_Hsap VKLFAVEET---------GGSRQKVLVMEYCSSGSLLS-VLESPENAFGLPEDEFLVVLR IKKb_Hsap VAARDVPEG-----MQNLAPNDLPLLAMEYCQGGDLRK-YLNQFENCCGLREGAILTLLS IKKa_Hsap VKACDVPEE-----LNI-LIHDVPLLAMEYCSGGDLRK-LLNKPENCCGLKESQILSLLS ird5_Dmel VAGVDIEDPDFLEYLNGMFSAKLPVIVLEYCNGGDVRK-RLQSPENANGLTEFEVRQILG ik2_Dmel VKLLAIEED---------QEGRGKVIVMELCTGGSLFN-ILDDPENSYGLPEHEFLLVLE IKKe_Mmus VKLFAVEET---------GGSRQKVLIMEYCSSGSLLS-VLEDPENTFGLSEEEFLVVLR IKKb_Mmus VAARDVPEG-----MQNLAPNDLPLLAMEYCQGGDLRR-YLNQFENCCGLREGAVLTLLS IKKa_Mmus VKACDVPEE-----LNF-LINDVPLLAMEYCSGGDLRK-LLNKPENCCGLKESQILSLLS TBK1_Mmus VKLFAIEEE---------TTTRHKVLIMEFCPCGSLYT-VLEEPSNAYGLPESEFLIVLR TBK1_Spur IHLDQIEED---------QISKQPVIVMELCTGGSLYT-YLDTPENLYGLKEKEFLQVLN IKKe_Spur VCLEAIEKD---------QISKENILIMELCTGGSLYD-VLEKPENSCGLAEKAFLQVLA IKKa_Spur VQGRPVPPE-----L-EAKPNEPPCLAMEFCSRGDLRK-KLSELPNMCGLPEAEILLLSK AqueK157_Aque IKALGPPPD-----IPYHPPEDVMHVWMEFCNGGDLRNGVLKKIENCCGVEEKQFRKICG AqueK158_Aque VSFFELNEI-----E---KKHQYHIIAMELCNGGSLYE-VIDSPQNAYGLDEYQFKSVIF : : :* * *.: :. * *: * . : TBK1_Hsap DVVGGMNHLRE-NGIVHRDIKPGNIMRVIGEDGQSVYKLTDFGAARELEDDEQFVSLYGT IKKe_Hsap CVVAGMNHLRE-NGIVHRDIKPGNIMRLVGEEGQSIYKLTDFGAARELDDDEKFVSVYGT IKKb_Hsap DIASALRYLHE-NRIIHRDLKPENIVLQQGE-QRLIHKIIDLGYAKELDQGSLCTSFVGT IKKa_Hsap DIGSGIRYLHE-NKIIHRDLKPENIVLQDVG-GKIIHKIIDLGYAKDVDQGSLCTSFVGT ird5_Dmel ALRKALHFLHSQCGICHRDLKPDNIVIQRGVDGKKIYKLTDFGLARGTPDQTMVQSVVGT ik2_Dmel HLCAGMKHLRD-NKLVHRDLKPGNIMKFISEDGQTIYKLTDFGAARELEDNQPFASLYGT IKKe_Mmus CVVAGMNHLRE-NGIVHRDIKPGNIMRLVGEEGQSIYKLSDFGAARKLDDDEKFVSVYGT IKKb_Mmus DIASALRYLHE-NRIIHRDLKPENIVLQQGE-KRLIHKIIDLGYAKELDQGSLCTSFVGT IKKa_Mmus DIGSGIRYLHE-NKIIHRDLKPENIVLQDVG-GKTIHKIIDLGYAKDVDQGSLCTSFVGT TBK1_Mmus DVVGGMNHLRE-NGIVHRDIKPGNIMRVIGEDGQSVYKLTDFGAARELEDDEQFVSLYGT TBK1_Spur DVSAGMKHLRD-KGIVHRDIKPGNIMMVKGEDGVIVYKLADFGAARELEDDEAFKSLYGT IKKe_Spur DMTAGMKYLRD-NGVVHRDVKPGNIMCFKDDHGHTIYKLADFGAARQLTDDENFTSLHGT IKKa_Spur HIASAMEYLHK-SDIIHRDIKPENIMVQD-WKGQC----------------------HGT AqueK157_Aque DINNGIRFLHK-ISVIHRDLKPENILIKHTDANTVVYKLTDFGYAKTYALSSKAQSLVGT AqueK158_Aque DVASGMRHLRD-HGFVHRDIKPGNIMRCINPDGSFVFKLADFGTARQLDPNEHFTSLHGT : .:..*:. . ***:** **: ** TBK1_Hsap EEYLHPDMYERAVLRKDHQKKYGATVDLWSIGVTFYHAATGSLPFRPFE-GPRRNKEVMY IKKe_Hsap EEYLHPDMYERAVLRKPQQKAFGVTVDLWSIGVTLYHAATGSLPFIPFG-GPRRNKEIMY IKKb_Hsap LQYLAPELLEQ--------QKYTVTVDYWSFGTLAFECITGFRPFLPNW-QPV---QWHS IKKa_Hsap LQYLAPELFEN--------KPYTATVDYWSFGTMVFECIAGYRPFLHHL-QPF---TWHE ird5_Dmel RHYYAPEVVEN--------GFYNSTVDLWSFGVIAYELVTGELPFIPHQ-TLK---NIIL ik2_Dmel EEYLHPDLYERAVLRKSIQRSFTANVDLWSIGVTLYHVATGNLPFRPFG-G-RKNRETMH IKKe_Mmus EEYLHPDMYERAVLRKPQQKAFGVTVDLWSIGVTLYHAATGSLPFIPFG-GPRRNKEIMY IKKb_Mmus LQYLAPELLEQ--------QKYTVTVDYWSFGTLAFECITGFRPFLPNW-QPV---QWHS IKKa_Mmus LQYLAPELFEN--------KPYTATVDYWSFGTMVFECIAGYRPFLHHL-QPF---TWHE TBK1_Mmus EEYLHPDMYERAVLRKDHQKKYGATVDLWSVGVTFYHAATGSLPFRPFE-GPRRNKEVMY TBK1_Spur EEYLHPDLYERAVLGKRNRTEFTAQTDLWSLGVTFFHTATGSLPFRAHG-G-RNNRDVMH IKKe_Spur EEYLHPKVFERAVLRKPAALPFDARIDLWSIGATLYHLATGQLPFRPHG-G-RKNSRMMF IKKa_Spur TQ----------------------TVDLVI------------------------------ AqueK157_Aque PQYVAPEIIDQ--------DSHGRPVDFWSFGVVAFELVTGIRPFLHKSRRPL---EWIG AqueK158_Aque EEYLYPAMYERALINYRSKEEFLDKVDLWSLGATFFHLATGRLPFRPYR-K-RQDRGTMF . * TBK1_Hsap KIITGKPSGAISGVQK-AE--NGPIDWSGDMPVSCSLSRGLQVLLTPVLANILEADQE-- IKKe_Hsap RITTEKPAGAIAGAQR-RE--NGPLEWSYTLPITCQLSLGLQSQLVPILANILEVEQA-- IKKb_Hsap KV-RQKSEVDIVVS-E-DL--NGTVKFSSSLPYPNNLNSVLAERLEKWLQLMLMWHPRQR IKKa_Hsap KI-KKKDPKCIFAC-E-EM--SGEVRFSSHLPQPNSLCSLVVEPMENWLQLMLNWDPQQR ird5_Dmel NL-IKKPAKCIAIT-E-DPEDNTRFVNQFELPQTHHLSRPWAAQFTKWLASPLNSNYKER ik2_Dmel QITTKKASGVISGTQL-SE--NGPIEWSTTLPPHAHLSQGLKTLVTPLLAGLLEENRE-- IKKe_Mmus RITTEKPAGAISGTQK-QE--NGPLEWSYSLPITCRLSMGLQNQLVPILANILEVEED-- IKKb_Mmus KV-RQKSEVDIVVS-E-DL--NGAVKFSSSLPFPNNLNSVLAERLEKWLQLMLMWHPRQR IKKa_Mmus KI-KKKDPKCIFAC-E-EM--TGEVRFSSHLPQPNSLCSLIVEPMESWLQLMLNWDPQQR TBK1_Mmus KIITGKPSGAISGVQK-AE--NGPIDWSGDMPLSCSLSQGLQALLTPVLANILEADQE-- TBK1_Spur RITTTKKSSMISGVQE-VP--NGPIIWSEDLPKTCHFSPDMRHQVKTLLSHTMECNIQ-- IKKe_Spur HLTTKKAPGVISGVQTGPL--DHEITWSRELPETCRLSIGLKKLITPLLAGLLESNPS-- IKKa_Spur ------------------------------------------------------------ AqueK157_Aque PT-RNKRDNCIYIT---FN--NGQLEYYDSIPSPHQLSSAFAGELTKWLQVVLHQESEKR AqueK158_Aque KIINEKKTGIILGWQQ-EY--NAPIYYSDRLPPDIVISQGLRDIITPILAGVLDTF-D-- TBK1_Hsap -----KCWGFDQFFAETSDILHRMVIHVFSLQQMTAHKIYIHSY---NTATIFHELVYKQ IKKe_Hsap -----KCWGFDQFFAETSDILQRVVVHVFSLSQAVLHHIYIHAH---NTIAIFQEAVHKQ IKKb_Hsap G--TDPTYGPNGCFKALDDILNLKLVHILNMVTGTIHTYPVTED---ESLQSLKARIQQD IKKa_Hsap GGPVDLTLKQPRCFVLMDHILNLKIVHILNMTSAKIISFLLPPD---ESLHSLQSRIERE ird5_Dmel G--QLAANNVPVVFADLDKILNMNVLTIFAVNNCERLEYAVSAE---MTMKDLIALIVLD ik2_Dmel -----KTWSFDRFFHEVTLILRKRVIHVFFTNRTSSVEVFLEPD---EQIDNFRERIFLQ IKKe_Mmus -----KCWGFDQFFAETSDILQRTVIHVFSLPQAVLHHVYIHAH---NTIAIFLEAVYEQ IKKb_Mmus G--TDPQYGPNGCFRALDDILNLKLVHVLNMVTGTVHTYPVTED---ESLQSLKTRIQED IKKa_Mmus GGPIDLTLKQPRCFALMDHILNLKIVHILNMTSAKIISFLLPCD---ESLHSLQSRIERE TBK1_Mmus -----KCWGFDQFFAETSDVLHRMVIHVFSLQHMTAHKIYIHSY---NTAAVFHELVYKQ TBK1_Spur -----KMWTFDIFFDFVQRLTSMRPVDVFVAPTCECHIVYVEPQ---RKVADFQDKMAHL IKKe_Spur -----TVWSFQHFFQASQAITTKIAIDVFWVCEASYMKIYCSPT---DRFAEFQENIASQ IKKa_Spur ---------------------------------HSPVTYPLDHD---TS----------- AqueK157_Aque G--VAHNGDEAPCLLHIDRILRTKVLIIVSMSNGHVLSYSVRCD---SSYQSLSAWLE-E AqueK158_Aque -----SMWAFDRFFKAIDGIKKQNVVHIFCVSRCTCHMIYVDDAGEADIIPKFKELIARQ TBK1_Hsap TKIISSNQELIYEGRRLVLEPGRLAQH-FPKTTEENPI---------FVVSREPL--NTI IKKe_Hsap TSVAPRHQEYLFEGHLCVLEPSVSAQH-IAHTTASSPL---------TLFSTAI--PKGL IKKb_Hsap TGIPEEDQELLQEAGL-ALIPDKPATQCISDGKLNEGHTLDM--DLVFLFDNSKITYET- IKKa_Hsap TGINTGSQELLSETGI-SLDPRKPASQCVLDGVR----GCDS--YMVYLFDKSKTVYEG- ird5_Dmel TGMDEKELYFVLPTSH---------PHK-TITPKSTPLQL----YVEEWSDTSKDS--R- ik2_Dmel TEVPLEKQILLFNNEHLE--KKV-----TPR-TIDQPI---------FLYSNDDNNV-QL IKKe_Mmus TNVTPKHQEYLFEGHPCVLEPSLSAQH-IAHTAASSPL---------TLFSMSSDTPKGL IKKb_Mmus TGILETDQELLQEAGL-VLLPDKPATQCISDSKTNEGLTLDM--DLVFLFDNSKINYET- IKKa_Mmus TGINTGSQELLSETGI-SLDPRKPASQCVLDGVR----GCDS--YMVYLFDKSKTVYEG- TBK1_Mmus TKIVSSNQELIYEGRRLVLELGRLAQH-FPKTTEENPI---------FVTSREQL--NTV TBK1_Spur TGIQPETQSLLWDKEVFDLKKCLKCED-LPQTSPDNPL---------ILVRMGAEVF--P IKKe_Spur TKLSATKQSILYNGETFKPDGILPISS-YPITSVANPI---------TLSNKEQIHV-QH IKKa_Spur ------------------------------------------------------------ AqueK157_Aque KGYPKSKQYLVTDRGE-LLSDSLLDLH-S-------SLRWDDCICTVYLFSQDVV-PSF- AqueK158_Aque TSLDPKHMEMYLENILFGTEPVR-ARD-LPLTTPDSPL---------TVFGGPPQKP--D TBK1_Hsap GLIYEK-ISLPKVHPRYDLDGDASMAKAITGVVCYACRIASTLLLY----QELMRKGIRW IKKe_Hsap AFRDPA-LDVPKFVPKVDLQADYNTAKGVLGAGYQALRLARALLDG----QELMFRGLHW IKKb_Hsap QISPRPQPESVSCILQE-----PKRNLAFF--------------------QLRKVWGQVW IKKa_Hsap PFASRSLSDCVNYIVQD-----SKIQLPII--------------------QLRKVWAEAV ird5_Dmel KWTKRSNPPVMLYIFQVKKECDYKIPEPILSILSRKF-IANKFKTKERWLQKRVVLDMLY ik2_Dmel PQQLDL-PKFPVFPPNVSVENDASLAKSACSVGHECKRRVDIFTSM----DILIKKGVEH IKKe_Mmus AFRDPA-LDVPKFVPKVDLQADYSTAKGVLGAGYQALWLARVLLDG----QALMLRGLHW IKKb_Mmus QITPRPQPESVSCILQE-----PKRNLSFF--------------------QLRKVWGQVW IKKa_Mmus PFASRSLSDCVNYIVQD-----SKIQLPII--------------------QLRKVWAEAV TBK1_Mmus GLRYEK-ISLPKIHPRYDLDGDASMAKAVTGVVCYACRTASTLLLY----QELMRKGVRW TBK1_Spur SVKVPSLPTQPKTATHFSVQQDSTLAKHIFTVVL-------------------------- IKKe_Spur QLTPPFVATMPALRPRFSTQNDYPIAKGALATGCILQTRQKMIHEM----HGLFDQYVIT IKKa_Spur --------------------ND-------------------------------------- AqueK157_Aque KLVVQVTP-SVTNLITN-----GDENLHRE--------------------DKYMHFAHAY AqueK158_Aque KLATPVVTQPPNMPPETTMVDDLRYAKDCTKAIWVMYRAAETMDRY----FRLMNQGALC TBK1_Hsap -LIELIKDDYNETVHKKTEVV---ITLD-----------------FCIR----------- IKKe_Hsap -VMEVLQATCRRTLEVARTSL---LYLS-----------------SSLG----------- IKKb_Hsap HSIQTLKEDCNRLQQGQRAAM---MNLL-------------RNN-SCLSKMKNSMASMSQ IKKa_Hsap HYVSGLKEDYSRLFQGQRAAM---LSLL-------------RYN-ANLTKMKNTLISASQ ird5_Dmel V-LTKEQARYEMLVSGINERA---LSLEDEMMENSFIDSIDKQR-IIISFAYDQLTSLLK ik2_Dmel -FIEMLVTTITLLLKKTESFD---NLLS-----------------TVID----------- IKKe_Mmus -VLEVLQDTCQQTLEVTRTAL---LYLS-----------------SSLG----------- IKKb_Mmus HSIQTLKEDCNRLQQGQRAAM---MSLL-------------RNN-SCLSKMKNAMASTAQ IKKa_Mmus HYVSGLKEDYSRLFQGQRAAM---LSLL-------------RYN-ANLTKMKNTLISASQ TBK1_Mmus -LVELVKDDYNETVHKKTEVV---ITLD-----------------FCIR----------- TBK1_Spur ------RMDYMKLRSEHSAVKSFCLQAD-----------------CRWR----------- IKKe_Spur -LRDRSKMDVLRLQDHHKLVKGKLSIMS-----------------THLG----------- IKKa_Spur ------------------------------------------------------------ AqueK157_Aque SHIKDQERLFWRIHNGHRA-I---LQLI-------------RPVTEELKGHYDDHSTEVA AqueK158_Aque -LRETLDKDYRAWCRKYEEVQ---VRFN-----------------SL------------- TBK1_Hsap -----------NIEKTVKVYEKLM----------K---INLEAAE-L---GEISDIHT-- IKKe_Hsap ----------------TERFSSVA--GTPEI---Q---ELKAAAE-L--RSRLRTLAE-- IKKb_Hsap QLKAKLDFFKTSIQIDLEKYSEQTEFGITSDKLLLAWREMEQAVELCGRENEVKLLVE-- IKKa_Hsap QLKAKLEFFHKSIQLDLERYSEQMTYGISSEKMLKAWKEMEEKAIHYAEVGVIGYLED-- ird5_Dmel EAQAKIPSRQLISSAQWEKLNRNYNFIIQSAKSIRSFL------EACLREA--KDMVK-- ik2_Dmel ------------YADVVHSMARVT----------K------G-------DQEIKTLLT-- IKKe_Mmus ----------------TERFSSGA--GMPDV---Q---ERKEATE-L--RTRLQTLSE-- IKKb_Mmus QLKAKLDFFKTSIQIDLEKYKEQTEFGITSDKLLLAWREMEQAVEQCGRENDVKHLVE-- IKKa_Mmus QLKAKLEFFRKSIQLDLERYSEQMTYGISSEKMLKAWKEMEEKAIHYSEVGVIGYLED-- TBK1_Mmus -----------NIEKTVKVYEKLM----------K---VNLEAAE-L---GEISDIHT-- TBK1_Spur -----------ALERSLT------------------------------------------ IKKe_Spur -----------DGK-------RIL--NL------------LRNTQ-E---GRISDITH-- IKKa_Spur ---------------------------------------------TCIYK---------- AqueK157_Aque KCLGVANFMKKMYSEDMEKLSDN--------SRINAWRSELS------------------ AqueK158_Aque -----------------------I--DI------------SNSQI-V---GLQSDVEFLQ TBK1_Hsap K-----L-----LRLSSSQG---------------------TIETSLQDIDSRLSPGGS- IKKe_Hsap V-----L-----SRCSQNIT---------------------ETQESLSSLNREL------ IKKb_Hsap R-----M-----MALQTDIVDLQRSPM--------------------------------- IKKa_Hsap Q-----I-----MSLHAEIMELQKSPY--------------------------------- ird5_Dmel TTNQLRK-----EVCEKDLFDCARFYK--------------------------------- ik2_Dmel A-----L-----ENVKSDFD---------------------GAADVISQMHKHFVIDDE- IKKe_Mmus I-----L-----SKCSHNVT---------------------ETQRSLSCLGEEL------ IKKb_Mmus R-----M-----MALQTDIVDLQRSPM--------------------------------- IKKa_Mmus Q-----I-----MSLHTEIMELQKSPY--------------------------------- TBK1_Mmus K-----L-----LRLSSSQG---------------------TIESSLQDISSRLSPGGL- TBK1_Spur -------------LVLTGVD-----------------------GASLDEIKLRII----- IKKe_Spur --------------------QLERKLEEGSHDLMQFEMQSNEIDEILNQLHTDVFINDI- IKKa_Spur ------------------------------------------------------------ AqueK157_Aque ------------------------------------------------------------ AqueK158_Aque A-----INPLCNNAFDHDIADLQRQLEDKNKHLVDVRDQSFALHEQTKDFHSTVN--SIT TBK1_Hsap LADAWA---HQEGTHPKD-RNVEKL---QVLLNCMTEIYYQFKKDKAERRLAYN--EEQI IKKe_Hsap ----VK---SRDQVHED--RSIQQI---QCCLDKMNFIYKQFKKSRMRPGLGYN--EEQI IKKb_Hsap ----------------GR-KQGGTL---DDLEEQARELYRRLREKPRDQRTEG-DSQEMV IKKa_Hsap ----------------GR-RQGDLM---ESLEQRAIDLYKQLKHRPSDHS-YS-DSTEMV ird5_Dmel ----------------KY-LCNGAIISPSELNNDAE----EFAKSRF-KL----YNEGEA ik2_Dmel LNDQWTSSMHGKKCPCKT-RASAQA---KYLVERLRDSWQHLLRDRATRTLTYN--DEQF IKKe_Mmus ----LK---NRDQIHEDN-KSIQKI---QCCLDKMHFIYKQFKKSRMRPGLSYN--EEQI IKKb_Mmus ----------------GR-KQGGTL---DDLEEQARELYRRLREKPRDQRTEG-DSQEMV IKKa_Mmus ----------------GR-RQGDLM---ESLEQRAIDLYKQLKHRPPDHL-YS-DSTEMV TBK1_Mmus LADTWA---HQEGTHPRD-RNVEKL---QVLLNCITEIYYQFKKDKAERRLAYN--EEQI TBK1_Spur -----K---CKERLYE--------V---QNVF---HSIFYV------------------- IKKe_Spur LQSIWKP---SQGCNVQDEKCSTEL---VHLTDEMEDIFKRFSRDKKTGKLSYN--EEQI IKKa_Spur ------------------------------L----------------------------- AqueK157_Aque ------------------------------------------------------------ AqueK158_Aque SSSIWDT---NYLAKVFN-ESTKTM---MSYVDKVEEIFNAFQEQKKQRGAIHGDYDRRS TBK1_Hsap HKFDKQKLYY-----------------HATKAMTH--FTD---ECVKKY----EAFLN-- IKKe_Hsap HKLDKVNFSH-----------------LAKRLLQV--FQE---ECVQKY----QASLV-- IKKb_Hsap -RLLLQAIQS-----------------FEKKVRVIYTQLSKTVVCKQKA----LELLPKV IKKa_Hsap -KIIVHTVQS-----------------QDRVLKELFGHLSKLLGCKQKI----IDLLPKV ird5_Dmel -----------------------------RHLPKSIDHMHYLY----------------- ik2_Dmel HALEKIKVDH-----------------NGKRIKAL--LLD---NVNPTV----AQIAE-- IKKe_Mmus HKLDKVNFSH-----------------LAKRLLQV--FQE---ECVQTY----QVSLV-- IKKb_Mmus -RLLLQAIQS-----------------FEKKVRVIYTQLSKTVVCKQKA----LELLPKV IKKa_Mmus -KIIVHTVQS-----------------QDRVLKELFGHLSKLLGCKQKI----IDLLPKV TBK1_Mmus HKFDKQKLYY-----------------HATKAMSH--FSE---ECVRKY----EAFKD-- TBK1_Spur ------------------------------------------------------------ IKKe_Spur HKMEKLSLGD-----------------LMKRTMTC---SG---GCEERWRSQHKLLMV-- IKKa_Spur ------------------------------------------------------------ AqueK157_Aque ----------------------------------------------------------KL AqueK158_Aque HEYNKRTIKERLGMADKKLSEMIAGRNHVYGNLYH--FLE---KCTP----VRE------ TBK1_Hsap -------KSEE-WIRK---MLHLRKQL-------LSL-------TNQCFD--IEEEVSKY IKKe_Hsap -------THGK-RMRV---VHETRNHL-------RLV-------GCSVAA--CNTEAQGV IKKb_Hsap EEVVSLMNEDEKTVVR-L-QEKRQKELW------NLL-------KIACSK--VRGPVSGS IKKa_Hsap EVALSNIKEADNTVMF-M-QGKRQKEIW------HLL-------KIACTQSSARSLVGSS ird5_Dmel --------------------FKTKESIP------VLL-------QQFCD---IKKEIFQI ik2_Dmel -------CLAD-WYKLAQ-TVYLKTQI---------L-------EKDVRD--CERKLNGI IKKe_Mmus -------THGK-RMRQ---VQRAQNHL-------HLI-------GHSVAT--CNSEARGA IKKb_Mmus EEVVSLMNEDERTVVR-L-QEKRQKELW------NLL-------KIACSK--VRGPVSGS IKKa_Mmus EVALSNIKEADNTVMF-M-QGKRQKEIW------HLL-------KIACTQSSARSLVGYS TBK1_Mmus -------KSEE-WMRK---MLHLRKQL-------LSL-------TNQCFD--IEEEVSKY TBK1_Spur -----------------------------------------------------EK----- IKKe_Spur -------CHNK-AYNQRQQLLKADRSLAPLVHHCNVLENTISDLHSQCWQ--IAESKTPP IKKa_Spur -----------------------------------------------AN---WEREILKF AqueK157_Aque D----------------------------------------------------------- AqueK158_Aque ------------------DLRNLMASLDHSAQCT---KEDTIRLQ---------KWLPHH TBK1_Hsap QEYT---NELQETLPQKMFTA-SSGIKHTMTP---------------------------- IKKe_Hsap QESL---SKLLEELSHQLLQDRAKGAQASPPP---------------------------- IKKb_Hsap PDSM---NASRLSQPGQLMSQ-PSTASNSL------------------------------ IKKa_Hsap LEGAVTPQTSAWLPPTSAEHD-HSLSCVVT------------------------------ ird5_Dmel NLQML--MSASSTPPPKLE----------------------------------------- ik2_Dmel RDEL---YHVKSELKLDVDTK-TINNNNQLAK---------------------------- IKKe_Mmus QESL---NKIFDQLLLDR--ASEQGAEVSPQP---------------------------- IKKb_Mmus PDSM---NVSRLSHPGQLMSQ-PSSACDSL------------------------------ IKKa_Mmus LEGTVTPPVSAWLPPTLADRE-HPLTCVVT------------------------------ TBK1_Mmus QDYT---NELQETLPQKMLAA-SGGVKHAMAP---------------------------- TBK1_Spur ------------------------------------------------------------ IKKe_Spur PDMK---RHHTYQAPKEILL-SASTQ-TSLPKEETPSHHTSLLLDHHYDQLTKGGKPLVM IKKa_Spur ------------------------------------------------------------ AqueK157_Aque ------------------------QAIKVH------------------------------ AqueK158_Aque QQRM---QNLLGKLPNSL-S-GGGG------------------------------GGGGM TBK1_Hsap -----------------------IYP-------------------------S-SNTLVE- IKKe_Hsap -----------------------IAP-------------------------YPSPTRKD- IKKb_Hsap -------------------------P-------------------------EPAKKSEE- IKKa_Hsap -------------------------P-------------------------QDGETSAQ- ird5_Dmel ------------------------------------------------------------ ik2_Dmel ---------------------------------------------------------IE- IKKe_Mmus -----------------------MAP-------------------------HPGPDPKD- IKKb_Mmus -------------------------P-------------------------ESDKKSEE- IKKa_Mmus -------------------------P-------------------------QDGETLAQ- TBK1_Mmus -----------------------IYP-------------------------S-SNTLVE- TBK1_Spur ------------------------------------------------------------ IKKe_Spur GKSTINSQRSPYSVDRSTGQYPELYQTASALSEPSCGTSQYRYDAPLTRARSSPPELVAR IKKa_Spur ------------------------------------------------------------ AqueK157_Aque -------------------------P-------------------------HSSAIST-- AqueK158_Aque GTLQLN------------------------------------------------------ TBK1_Hsap -------------------------------------------MTLGMKKLKE------- IKKe_Hsap -------------------------------------------LLLHMQELCE------- IKKb_Hsap -------------------------------------------LVAEAHNLCT---LLEN IKKa_Hsap -------------------------------------------MIEENLNCLG---HLST ird5_Dmel -------------------------------------------LSAAMDRLAISSGSPSS ik2_Dmel -------------------------------------------ERNRLRVMQQ------- IKKe_Mmus -------------------------------------------LVFHMQELCN------- IKKb_Mmus -------------------------------------------LVAEAHALCS---RLES IKKa_Mmus -------------------------------------------MIEENLNCLG---HLST TBK1_Mmus -------------------------------------------MTLGMKKLKE------- TBK1_Spur ------------------------------------------------------------ IKKe_Spur GISRRSPDAPSELYGVDPTPGAIGGLQDLRSPAGDYQQAEGPRMIKVSKEFKK------- IKKa_Spur ------------------------------------------------------------ AqueK157_Aque -------------------------------------------HTHTHTHTCR---TFIH AqueK158_Aque ------------------------------------------------------------ TBK1_Hsap EMEGVVKELAENNHI-------------------------------LERFGSLTMD---- IKKe_Hsap GMKLLASDLLDNNRI-------------------------------IERLNRV------- IKKb_Hsap AIQDTVREQDQSFTA-------------------------------LDW-SWLQTEE--- IKKa_Hsap IIHEANEEQGNSMMN-------------------------------LDW----------- ird5_Dmel DPFDS-----------------------------------------------LRTIN--- ik2_Dmel QQQEVMAVMRTNSDI-------------------------------ISLLSKLGIT---- IKKe_Mmus DMKLLAFDLQDNNRL-------------------------------IERLHRV------- IKKb_Mmus ALQDTVKEQDRSFTT-------------------------------LDW-SWLQMED--- IKKa_Mmus IIREANEDQSSSLMS-------------------------------LDW----------- TBK1_Mmus EMEGVVKELAENNHI-------------------------------LERFGSLTMD---- TBK1_Spur ------------------------------------------------KF--LKE----- IKKe_Spur RVADYRKESNTSYHIQRNLAELTQLELESMSLETPEMPRYTADSSLLDTLTGPGMDEGDE IKKa_Spur ------------------------------------------------------------ AqueK157_Aque ----------------------------------------------------LLL-R--- AqueK158_Aque -----------------SLRSLLQRAREST----GDLL------KLADKQ---------- TBK1_Hsap ----------GGLRNVDCL IKKe_Hsap -------------PAPPDV IKKb_Hsap --------EEH-SCLEQAS IKKa_Hsap --------------SWLTE ird5_Dmel --------AIEEAERINNM ik2_Dmel ----------N--GSLESS IKKe_Mmus -------------PSAPDV IKKb_Mmus --------EERCSLEQACD IKKa_Mmus --------------SWLAE TBK1_Mmus ----------GGLRNVDCL TBK1_Spur ----------EG-SHMLLN IKKe_Spur SILEGLDIKRPPVSYQSGT IKKa_Spur ----------MGSMYNARL AqueK157_Aque --------CMHFPKRLERS AqueK158_Aque KTL-----IHPPSDKKEKS