Difference between revisions of "Kinase Subfamily HYKK"

From WikiKinome
Jump to: navigation, search
m (Gerard moved page Kinase Subfamily AGPHD1 to Kinase Subfamily HYKK: Gene and family renamed after function discovered)
m
Line 1: Line 1:
 
__NOTOC__
 
__NOTOC__
[[kinase classification|Kinase Classification]]: [[Kinase_Group_PKL|Group PKL]]: [[Kinase_Family_CAK|Family CAK]]: [[Kinase_Subfamily_AGPHD1|Subfamily AGPHD1]]
+
[[kinase classification|Kinase Classification]]: [[Kinase_Group_PKL|Group PKL]]: [[Kinase_Family_CAK|Family CAK]]: [[Kinase_Subfamily_AGPHD1|Subfamily HYKK]]
  
AGPHD1 is a eukaryotic kinase recently shown to phosphorylate a lysine derivative during collagen catabolism
+
HYKK is a eukaryotic kinase recently shown to phosphorylate a lysine derivative during collagen catabolism
  
 
====Evolution====
 
====Evolution====
AGPHD1 is found in in most animals and some bacteria.
+
HYKK is found in in most animals and some bacteria.
  
 
====Function====
 
====Function====
Human AGPHD1 was recently shown to phosphorylate 5-hydroxy-lysine, as part of the breakdown process for collagen (collagen has a unique post-translational modification of some lysines to form 5-hydroxy-lysine, and so requires a specific degradation mechanism) <cite>Veiga-da-Cunha</cite>. Its role was elegantly revealed using a phylogenetic tip: AGPHD1 is also found in some bacteria, where it frequently is fused with homologs of AGXT2L1/2. Biochemical analysis showed AGPHD1 to be a GTP-dependent kinase of 5-hydroxy-lysine, and AGXT2L2 to carryout an aminotransferase reaction on the phosphorylated 5-hydoxy-lysine. This function is reminiscent of [[Kinase_Family_FruK|Fructosamine Kinase]], which phosphorlyates glycated residues on aged proteins, providing the phosphate energy for a later deglycation step.
+
Human HYKK was recently shown to phosphorylate 5-hydroxy-lysine, as part of the breakdown process for collagen (collagen has a unique post-translational modification of some lysines to form 5-hydroxy-lysine, and so requires a specific degradation mechanism) <cite>Veiga-da-Cunha</cite>. Its role was elegantly revealed using a phylogenetic tip: HYKK is also found in some bacteria, where it frequently is fused with homologs of AGXT2L1/2. Biochemical analysis showed HYKK to be a GTP-dependent kinase of 5-hydroxy-lysine, and AGXT2L2 to carryout an aminotransferase reaction on the phosphorylated 5-hydoxy-lysine. This function is reminiscent of [[Kinase_Family_FruK|Fructosamine Kinase]], which phosphorlyates glycated residues on aged proteins, providing the phosphate energy for a later deglycation step.
  
 
====Domain Structure====
 
====Domain Structure====
Most animal AGPHD1 have a sole domain, the kinase domain. This domain conserves the key catalytic motifs of the PKL fold, and is similar to both the CAK and HSK2 families of small molecule kinases.
+
Most animal HYKK have a sole domain, the kinase domain. This domain conserves the key catalytic motifs of the PKL fold, and is similar to both the CAK and HSK2 families of small molecule kinases.
  
 
====Evolution====
 
====Evolution====

Revision as of 23:26, 18 January 2017

Kinase Classification: Group PKL: Family CAK: Subfamily HYKK

HYKK is a eukaryotic kinase recently shown to phosphorylate a lysine derivative during collagen catabolism

Evolution

HYKK is found in in most animals and some bacteria.

Function

Human HYKK was recently shown to phosphorylate 5-hydroxy-lysine, as part of the breakdown process for collagen (collagen has a unique post-translational modification of some lysines to form 5-hydroxy-lysine, and so requires a specific degradation mechanism) [1]. Its role was elegantly revealed using a phylogenetic tip: HYKK is also found in some bacteria, where it frequently is fused with homologs of AGXT2L1/2. Biochemical analysis showed HYKK to be a GTP-dependent kinase of 5-hydroxy-lysine, and AGXT2L2 to carryout an aminotransferase reaction on the phosphorylated 5-hydoxy-lysine. This function is reminiscent of Fructosamine Kinase, which phosphorlyates glycated residues on aged proteins, providing the phosphate energy for a later deglycation step.

Domain Structure

Most animal HYKK have a sole domain, the kinase domain. This domain conserves the key catalytic motifs of the PKL fold, and is similar to both the CAK and HSK2 families of small molecule kinases.

Evolution

Found in most or all animals, in some other eukaryotes (algae and some heterokonts) and in some bacteria. Bacterial forms are frequently fused to an OAT aminotransferase domain and a single fungal homolog (XP_384884.1 from Gibberella zeae) has the same domain arrangement, probably a case of horizontal transfer.

References

  1. Veiga-da-Cunha M, Hadi F, Balligand T, Stroobant V, and Van Schaftingen E. Molecular identification of hydroxylysine kinase and of ammoniophospholyases acting on 5-phosphohydroxy-L-lysine and phosphoethanolamine. J Biol Chem. 2012 Mar 2;287(10):7246-55. DOI:10.1074/jbc.M111.323485 | PubMed ID:22241472 | HubMed [Veiga-da-Cunha]