Gene GCN2 (Mouse)

GCN2

Species: Mouse
Alias: GCN2, Eif2ak4
External Links: MGI , Entrez Gene , Refseq cDNA , Refseq Protein , Phosphosite , iHOP
Annotation: Secondary Group: STE, Secondary Family: STE-Unique,

Classification

Group: Other
Family: PEK
Subfamily: GCN2

Sequence

Name Sequence Type Origin Length Description Download
GCN2.kin_dom Protein Kinase Domain Sugen global kinase HMM prediction 412 Kinase domain by global Sugen HMM Fasta, JSON
GCN2.NA RNA Sugen 5212 None Fasta, JSON
GCN2.AA Protein Sugen 1648 None Fasta, JSON
GCN2.kin_dom2 Protein Kinase Domain Sugen global kinase HMM prediction 259 Kinase domain by global Sugen HMM Fasta, JSON

Protein domain

Protein domains of GCN2.AA. The domains are annotated by HMM profiles from Pfam and SMART, as well as in-house data which includes HMMs of each individual kinase group, family and subfamily. Here is domain list in details, including sequence identity, significance and alignment. In particular, you can find can find the best hit kinase group/family/subfamily, which is helpful to understand the relationship between kinases.Kinase domain and best hit kinase group/family/subfamily are highlighted in red and blue. Visualized by pviz.

Usage: Zoom in selected region by dragging mouse and zoom out by double-clicking.

Show/hide domain list
Domain Protein name Domain Name Range Identity (%) Significance Score Profile Source Profile Range (length) Alignment
RWD GCN2.AA RWD 25-137 34 7.2e-41 143.02 SMART 1-128 (128) Show / Hide 
Range on Protein: 25-137
Range on HMM: 1-128/128
Sequence Identity: 34% (45 aa)

HELQALEAIYGSDFQDLRPDARGRVREPPEINLVLYPQG------------LAGEEVYVQVELRVKCPPTYPDVVPEIDLKNAK-GLSNESV--NLLKSH
.|| |||.||..||.... |....| ....| ... |.             . ||. |....| ..||..|||..|.|.|.| . .||.| .  . |..|
MELEALESIYPDDFECIDEDRHHWV-INCCIQIHIHPHEDEMQITNYDNFYE-GEDHYWCITLHFHCPENYPDEAPPIFLENPQKWLSDEQKYYQELQKH

LEELAKKQ--CGEVMIFELAHHVQSFLSEH
|.|.....  .|..||||....||.|||||
LQEIWEENWCRGMCMIFEWVQWVQEFLSEH
RWD GCN2.AA RWD 18-134 28 6.7e-33 120.43 Pfam 1-119 (119) Show / Hide 
Range on Protein: 18-134
Range on HMM: 1-119/119
Sequence Identity: 28% (35 aa)

SYSQRQDHELQALEAIYGSDFQDLRPDARGRVREPPEINLVLYPQGLAGEEVYVQVELRVKCPPTYP-DVVPEID-LKNAKGLS---NESVNLLKSHLEE
 . ..|. ||.|||.||. ||.|||. .   . .|..... .     .... .. ..| ..||..|| |..|.|. ... ..|.   .. .. |...|.|
MCQEEQEDELEALESIYPDDFEDLRGTICWTEISPWHFTIKI---DECESSHHPPLVLHFQCPEDYPSDEPPIISIISC-WNLHKLPDYQIQQLCKKLQE

LAKKQC-GEVMIFELAHHVQSFL
... .| |.|||||.. ..| .|
YWEENCHGMVMIFEWIEWLQENL
Kinase GCN2.AA GCN2 352-538 23 3.4e-81 276.04 In-house 260-511 (511) Show / Hide 
Range on Protein: 352-538
Range on HMM: 260-511/511
Sequence Identity: 23% (59 aa)

LSHPNIVRYFAMNSREEEDSIVIDILAEHVSGISLATHLSHSGPVPAHQ-LRKYTAQLLAGLDYLHSNSVVHKVLSASSVLVDAEGTVKITDYSISKR--
..|.|.|.|..||...........|. |...| .|.......|....|. |..|..|.| ||.|.||....|..|..| ...|..|.|||.|......  
NQHKNKVHYKCMNCKKPKEVHYLYIQMEYCEGKTLRQYIDNHGWCMDHDELWRYFRQILEGLNYIHSQGMIHRDLKPSNIFLDCCGNVKIGDFGLATNNK

----------------------LADICKEDVFEQARVRFSDS-------ALPYKTG---------------KKGDVWRL-GLLLLSLSQGQECGEYP---
                      ...||..|.|.|....||..       ....|.|               .|.|...| |...........|....   
MESQINRKQQKQQQQASSHWAIQNEICQDDQFNQQQSQFSQWDQQDEQVHHTGKVGTPFYMCPEQQKSHYNQKVDMYSLGGIIFFEMWYPPFCTQMERVQ

-------VTIPSDLP-------ADFQDFLKKCVCLDDKERWSPQQLLKHSFI
       ...|.|..       .|.||....|...|...|.|.||||.|.|.
ILKQLRSCEFPQDFDKKVDNHHKDQQDMIRWCLNHDPQKRPSAQQLLQHEFL
Kinase GCN2.AA PEK 372-538 13 5.6e-45 154.27 In-house 356-655 (655) Show / Hide 
Range on Protein: 372-538
Range on HMM: 356-655/655
Sequence Identity: 13% (40 aa)

IVIDILAEHVSGISLATHLSHSG--------------------------------------------PVPAHQLRKYTAQLLAGLDYLHSNSVVHKVLSA
..  |. |.... .| ......|                                             ....|......|.| ||.|.||. ..|. | .
HYLYIQMEYCEKKTLRDWIDNNGCPVQFNYWRRNSCKNHYNNYIMNQCCFQSTWSMNMRQWDENNCMFWDKDQCWRIFRQILEGLNYIHSQGMIHRDLKP

SSVLVDAEGT-----VKITDYSISKR----------------------LADICKEDVFEQA-----------------------------------RVRF
| ...| .|.     ||| |......                      ..| |......|                                    ....
SNIFLDCDGQHHQMQVKIGDFGLATNHEMLSQSQKNYNQQDQSYWKYVANDNCQWWNTNQQNSPTGQDTHMWSNNYHIQPTSMFYCEHCHHTGGVGTCLY

SDS-ALPYK------TGKKGDVWRLGLLLLSLSQGQECGEYPVTIPSDLPAD--------------------FQDFLKKCVCLDDKERWSPQQLLKHSFI
..   |...       ..|.|.. ||............ ... ... . . |                    ..||.......|...|.|.||.|.| |.
MSPEQLSGQHYQKSHYNHKVDMYSLGIIFFEMWYPFFTTMMERVKTLTDLRDESIQFPGDFPPKWFDNKYPKQADFIQWMLSHDPQQRPSAQQILQHEFF
Kinase GCN2.AA PEK 280-370 30 1.4e-30 103.26 In-house 1-89 (655) Show / Hide 
Range on Protein: 280-370
Range on HMM: 1-89/655
Sequence Identity: 30% (28 aa)

VHKGRCVGSDEQLGKVVYNALETATGSFVLLHEWVLQWQK-MGPCLTSQEKEKIDKCKRQIQGAETEFSSLVKLSHPNIVRYFAMNSREEED
.....|.|.... | .||.. . . | .  .....|.|.. ..||.| ||.. |.||| ... .. |...|..|.|||||||....  ..|.
FEEIQCLGKGGF-GGQVYKVQNKLDGCYYAIKRIKLNWKQRKQPCIT-QECM-IHKCKKHCEKVLREVKTLARLNHPNIVRYHNAWIEKPEE
Kinase GCN2.AA GCN2 281-351 18 6.7e-23 76.71 In-house 1-72 (511) Show / Hide 
Range on Protein: 281-351
Range on HMM: 1-72/511
Sequence Identity: 18% (13 aa)

HKGRCVGSDEQLGKVVYNALETATGSFVLLHEWVLQWQK-MGPCLTSQEKEKIDKCKRQIQGAETEFSSLVK
... |.......|.||.|. ....| ......|...|.. ..||......|....|....|. .... ....
FEEICCLGKGGFGQVVKNVRNMHDGRYYAIKKWPINWKQRKLPCKHKIMREVKLLCRLHHQHVVRYYQAWIE
Kinase GCN2.AA Pkinase 331-422 25 1.2e-15 56.47 Pfam 31-131 (293) Show / Hide 
Range on Protein: 331-422
Range on HMM: 31-131/293
Sequence Identity: 25% (27 aa)

KIDKCKRQIQGAET-----EFSSLVKLSHPNIVRYFAMNSREEEDSIVIDILAEHVSGI----SLATHLS--HSGPV--PAHQLRKYTAQLLAGLDYLHS
.|.|...  .  .|     | . . .|.||||||.. .  .  .|.|   .  |.. |     .|....|   .|..  . .....|  |.| ||.|.||
IIKKRHEKSWKDQTHRRLSEIQIMKRLNHPNIVRLYDWFED--KDHIY--MVMEYCEGGLYVMDLFDYISTWRHGCFYFSEWECKFYMYQILRGLEYCHS

NSVVH
....|
MGIIH
Kinase GCN2.AA STE11 351-453 37 7.4e-15 50.4 In-house 66-171 (303) Show / Hide 
Range on Protein: 351-453
Range on HMM: 66-171/303
Sequence Identity: 37% (40 aa)

KLSHPNIVRYFAMNSREEEDSIVIDILAEHVSGISLATHLS-HSGP--VPAHQLRKYTAQLLAGLDYLHSNSVVHKVLSASSVLVDAEGT-VKITDYSIS
.|.|.|||||.    .. ... |  |  |...| ||.  |.   ||   . .  |||| |.| || ||||. ..|. . .  .|.|..|. .|..|  ..
HLNHKNIVRYYGCCLHDNQNDHVN-IFMEYCPGGSLSSMLRHKWGPMKFDEMVIRKYTKQILEGLQYLHSHGIIHRDIKGANILLDSDGNVCKLADFGCA

KRLADIC
|||| ||
KRLAWIC
Kinase GCN2.AA STE 331-451 21 1.7e-13 44.17 In-house 48-199 (359) Show / Hide 
Range on Protein: 331-451
Range on HMM: 48-199/359
Sequence Identity: 21% (34 aa)

KIDKCK----------------RQIQGAETEFSSLVKLS--HPNIVRYFAMNSRE---EEDSIVIDILAEHVSGISLATHLSHS--------------GP
....|.                 ..|  . | . . |..  |||||.|. .  .|   ...   . |  |...| ||   . |               ..
NLENCNNKDENQENQELENKNHEEMQQILKEIKIMKKCNHMHPNIVKYYGCYFKEGHNHKNDDELWICMEYMDGGSLDDIIKHKYGCGKPLNHNRQHQHR

VPAHQLRKYTAQLLAG-LDYLHSNSVVHKVLSASSVLVDAEGTVKITDYSISKRLAD
.| .|..... |.| | |.||||. ..|  . .. .|.     ||..|  ....|.|
FPESQIAYICRQILKGLLYYLHSHHIIHRDIKPANILL-----VKLCDFGVCGQLTD
Kinase GCN2.AA S_TKc 286-538 18 2.6e-12 3.72 SMART 1-231 (231) Show / Hide 
Range on Protein: 286-538
Range on HMM: 1-231/231
Sequence Identity: 18% (53 aa)

VGSDEQLGK----VVYNALETATGSFV---LLHEWVLQWQKMGPCLTSQEKEKIDKCKRQIQGAETEFSSLVKLSHPNIVRYFAMNSREEEDSIVIDILA
    ...||    .|| . .. ||  |    ..                            . .. | . | |  |||||... .          . .  
YEILRKIGKGAFGKVYKCRHKKTGRIVAIKIIK----------------------------EHIRREIQILKK-HHPNIVKLYDVFQD-----DHLYMVM

EHVSG--ISLATHLSHSGP------VPAHQLRKYTAQLLAGLDYLHSNSVVHKVLSASSVLVDAEGTVKITDYSISKRLADICKEDVF---------EQA
|...|   .| ... . |.       |  . |.|..|.. .|.|.||. ..|  |  . .|.|   ..||.|  ....|      . |         |  
EYCDGDLGDLFDYIKKRGRHGLRFPFPE-HARFYMYQICCALEYCHSHGIIHRDLKPENILLDE--HIKICDFGLARQL------TTFCGTPWYMAPEVL

RVRFSDSALPYKTGK-KGDVWRLGLLLLSLSQGQE-----CGEYPVTIPSDLPADFQDFLKKCVCLDDKERWSPQQLLK-------HSFI
           . || | |.| .| .|  .  |.         . .      . .. ||..|| . | ..|..  ..|.       | ..
-----------GYGKCKCDWWSCGCILYEMLCGYPPFPQMQMMFKKIG----SPEAKDFIRKCLQKDPEKRPTA-EALQDEWDIKCHPWF
Kinase GCN2.AA STE 500-538 28 1.1e-11 37.68 In-house 300-359 (359) Show / Hide 
Range on Protein: 500-538
Range on HMM: 300-359/359
Sequence Identity: 28% (17 aa)

PVTIPSDLP-------------------ADFQDFLKKCVCLDDKERWSPQQLLK--HSFI
| ..|..|                     .|.||..||   | ..|....||||  |.||
PPPLPDHLSQNEMISYGGSGTTYPHKWSPEFKDFINKCLQKDPNKRPTASQLLKSEHPFI
Kinase GCN2.AA STE20 510-538 48 4.8e-10 33.19 In-house 260-288 (288) Show / Hide 
Range on Protein: 510-538
Range on HMM: 260-288/288
Sequence Identity: 48% (14 aa)

DFQDFLKKCVCLDDKERWSPQQLLKHSFI
.|.||..|| . | ..|....|||||.||
EFHDFVDKCLQKDPEKRPTASQLLKHPFI
Kinase GCN2.AA STE-Unique 379-456 23 2.5e-09 28.7 In-house 374-467 (616) Show / Hide 
Range on Protein: 379-456
Range on HMM: 374-467/616
Sequence Identity: 23% (22 aa)

EHVSGISLATHLSHSGP---------VPAHQLRKYTAQLLAGLDYLHSNS--VVHKVLSASSVLVDAEG-----TVKITDYSISKRLADICKED
|  .. .| . .  .|.          |. .  .|| |.| ||.||||    ..|  | .. .. . .|      ||| |. ...... . .|.
EYCEHGTLQQIIKRCGCPGNPNNLQLFPETEVIWYTYQILEGLEYLHSQHNPIIHRDLKPHNIFCMQDGKGRVCKVKICDFGHATCCQPDHFEK
Kinase GCN2.AA STE11 503-538 35 1.5e-08 27.36 In-house 262-303 (303) Show / Hide 
Range on Protein: 503-538
Range on HMM: 262-303/303
Sequence Identity: 35% (15 aa)

IPSDLP------ADFQDFLKKCVCLDDKERWSPQQLLKHSFI
||...       .. .|||..|   | |.|.. .|||.|.||
IPEHMSPISWISEECRDFLRHCFEHDPKKRPTANQLLQHPFI
Kinase GCN2.AA CAMK 329-422 17 3e-08 21.93 In-house 57-202 (425) Show / Hide 
Range on Protein: 329-422
Range on HMM: 57-202/425
Sequence Identity: 17% (25 aa)

KEKIDKCKR--------QIQGAETEFSSLVKLS-------HPNIVRYFAMNS------------REEE-----DSIVIDIL--AEHVSGI----SLATHL
| .. |||.          .  . | . . ||.       ||||.. . .                .      |. .. ..   |..||      |  ..
KIDKSKCKHRYCWRRPKDRKNVRREIEIMKKLQGIGNKLDHPNIIKLYEVYEDPHYFKNTNDYET-SKQVFTKDKHMYMVMEIMEYCSGGELFDDLFDYI

SHSGP---------------VPAHQLRKYTAQLLAGLDYLHSNSVVH
   |.               .. .. |.|  |.. ...|.||. .||
VEHGCYGEEFDQFDIVERGRFTEREARRYFRQICSAVHYCHSHNIVH
Kinase GCN2.AA STE20 407-442 44 8.8e-07 21.13 In-house 125-160 (288) Show / Hide 
Range on Protein: 407-442
Range on HMM: 125-160/288
Sequence Identity: 44% (16 aa)

QLLAGLDYLHSNSVVHKVLSASSVLVDAEGTVKITD
..| |||||||| ..|  . |  .|.. .| ||..|
EVLQGLDYLHSNHIIHRDIKADNILLTEDGEVKLAD
Kinase GCN2.AA Pkinase 507-538 34 2e-06 24.01 Pfam 262-293 (293) Show / Hide 
Range on Protein: 507-538
Range on HMM: 262-293/293
Sequence Identity: 34% (11 aa)

LPADFQDFLKKCVCLDDKERWSPQQLLKHSFI
.. ...||.|.| |.| |.|....|.|.| ..
WSEECKDFIKWCLCKDPKKRPTAEQILQHPWF
Kinase GCN2.AA CAMK 507-538 21 2e-06 15.86 In-house 394-425 (425) Show / Hide 
Range on Protein: 507-538
Range on HMM: 394-425/425
Sequence Identity: 21% (7 aa)

LPADFQDFLKKCVCLDDKERWSPQQLLKHSFI
. .. .|...|. . | ..|.. .| |.| ..
VSQECKDLIRKMLQVDPEKRMTIEQCLNHPWM
Kinase GCN2.AA Ciliate-E2 404-451 30 6e-06 16.34 In-house 165-208 (365) Show / Hide 
Range on Protein: 404-451
Range on HMM: 165-208/365
Sequence Identity: 30% (15 aa)

YTAQLLAGLDYLHSNS-VVHKVLSASSVLVDAEGTVKITDYSISKRLAD
| |... .| ||||.  ..|  .  . .|.     .||.|  .||.. |
YIACIILALEYLHSKNNIIHRDIKPENILF-----IKIIDFGLSKKYDD
Kinase GCN2.AA Ciliate-E2 503-538 19 2.2e-05 14.4 In-house 325-365 (365) Show / Hide 
Range on Protein: 503-538
Range on HMM: 325-365/365
Sequence Identity: 19% (8 aa)

IPSDLPADFQDFLKK----CVCLDDKERWS-PQQLLKHSFI
.. ... . .|..||    . . | ..| . ..|.|.| ..
SWKKWSQECKDLIKKFGKRMLQKDPEKRITGWEQILNHPWF
Kinase GCN2.AA Pkinase_Tyr 391-442 30 0.002956 11.65 Pfam 100-154 (270) Show / Hide 
Range on Protein: 391-442
Range on HMM: 100-154/270
Sequence Identity: 30% (17 aa)

SH---SGPVPAHQLRKYTAQLLAGLDYLHSNSVVHKVLSASSVLVDAEGTVKITD
.|    .|.. ..| .. .|  .|..||.|.  .|  | |  .||. ...|||.|
KHREDKQPLTMKDLLQMCWQIAKGMEYLESKNCIHRDLAARNCLVTENNVVKICD
Kinase GCN2.AA WEE 400-422 29 0.004371 6.28 In-house 112-135 (297) Show / Hide 
Range on Protein: 400-422
Range on HMM: 112-135/297
Sequence Identity: 29% (7 aa)

QLRKYTAQLLAGLDYLHSN-SVVH
.....  ... || ..||| . ||
MIWQILVDICQGLHHIHSNCGMVH
Kinase GCN2.AA STE-Unique 503-538 33 0.011266 4.65 In-house 581-616 (616) Show / Hide 
Range on Protein: 503-538
Range on HMM: 581-616/616
Sequence Identity: 33% (12 aa)

IPSDLPADFQDFLKKCVCLDDKERWSPQQLLKHSFI
||...  ..|.|. ||  ..   | |. |||.| . 
IPPKCQPEQQQFIEKCLERNPHHRPSAHQLLHHFWL
Kinase GCN2.AA PEK 351-361 72 0.022877 2.33 In-house 61-71 (758) Show / Hide 
Range on Protein: 351-361
Range on HMM: 61-71/758
Sequence Identity: 72% (8 aa)

KLSHPNIVRYF
.| |.||||||
MLDHHNIVRYF
Kinase GCN2.AA STE-Unique 345-360 43 0.038763 2.71 In-house 51-66 (616) Show / Hide 
Range on Protein: 345-360
Range on HMM: 51-66/616
Sequence Identity: 43% (7 aa)

EFSSLVKLSHPNIVRY
|. ....|.|.|||.|
EIEIMKCLHHQNIVKY
Kinase GCN2.AA STE20 331-369 23 0.061761 3.22 In-house 37-82 (288) Show / Hide 
Range on Protein: 331-369
Range on HMM: 37-82/288
Sequence Identity: 23% (11 aa)

KIDKCK---RQIQGAETEFSSLVKLSHPNIVRYFAMNSRE----EE
...||.    ...  ..| | . .. |||||.|.    ..    .|
NLEKCDNEQDEWEDIQKEISIMKQCKHPNIVKYYGSYLKDDTCDDE
Kinase GCN2.AA GCN2 589-1000 38 5.6e-223 747.05 In-house 1-511 (511) Show / Hide 
Range on Protein: 589-1000
Range on HMM: 1-511/511
Sequence Identity: 38% (197 aa)

FEELQLLGKGAFGAVIK-VQNKLDGCCYAVKRIPINPASR-HFRR--IKGEVTLLSRLHHENIVRYYNAWIERHERPAVPGTPPPDCTPQAQDSPATCGK
|||...||||.||.|.| |.|..||..||.|..|||...| ....  |..||.||.||||...||||.||||....|.|..|...|.|.....|......
FEEICCLGKGGFGQVVKNVRNMHDGRYYAIKKWPINWKQRKLPCKHKIMREVKLLCRLHHQHVVRYYQAWIEKCDEPMVTDTNNDDDTNNNYHSDDESEC

TSGDTEELGSVEAAAPPPILSSSVEWSTSAERSTSTRFPVTGQDSSSD----------------------------------------------------
.||..|.. ||...........|.|||.|.||..|.........|..|                                                    
FSGQYEMPKSVNQSEEMCNKEESMEWSMSNERNNSNWHDNGNFFSEWDNPFDQPFGPKCAFRMGQKRTFDLTDDHYSYCHSDCEFDDDVFDIDWDIESNN

------EEDEDERDGVFSQSFLPASDSDSDIIFDNEDEN---------SKSQNQDEDCNQKDGSHE---IEPSVTAEAVHYLYIQMEYCEKSTLRDTIDQ
      . .|.|......|||.|..........|.||.|         .|||.||..|... ....    ..... ..||||||||||||..|||..||.
VRDMESDDNEWEQEQQNNQSFQPKQEDHQNQYYDQEDLNNAESEDQNKKKSQQQDKMCQNQHKNKVHYKCMNCKKPKEVHYLYIQMEYCEGKTLRQYIDN

G-LFRDTSRLWRLFREILDGLAYIHEKGMIHRDLKPVNIFLDSDDHVKIGDFGLAT--------------------DHLAFTAEGKQDDQAGDGVIKSDP
. ...|. .|||.||.||.||.|||..|||||||||.|||||....||||||||||                    .|.|. .|..|||| .. ......
HGWCMDHDELWRYFRQILEGLNYIHSQGMIHRDLKPSNIFLDCCGNVKIGDFGLATNNKMESQINRKQQKQQQQASSHWAIQNEICQDDQFNQQQSQFSQ

SG------HLTGMVGTALYVSPEVQGSTKSAYNQKVDLFSL-GIIFFEMSYHPMVTASERIFVLNQLRDPTSPKFPDDFD---DGEHTKQKSVISWLLNH
..      |.||.|||..|..||.|   ||.||||||..|| |||||||.|.|..|..||...|.|||   |..||.|||   |..|..|...|.|.|||
WDQQDEQVHHTGKVGTPFYMCPEQQ---KSHYNQKVDMYSLGGIIFFEMWYPPFCTQMERVQILKQLR---SCEFPQDFDKKVDNHHKDQQDMIRWCLNH

DPAKRPTAMELLKSELL
||.|||.|..||..|.|
DPQKRPSAQQLLQHEFL
Kinase GCN2.AA PEK 589-1000 27 9.1e-190 636.73 In-house 1-655 (655) Show / Hide 
Range on Protein: 589-1000
Range on HMM: 1-655/655
Sequence Identity: 27% (183 aa)

FEELQLLGKGAF-GAVIKVQNKLDGCCYAVKRIPINPAS----------------RHFRRIKGEVTLLSRLHHENIVRYYNAWIERHERPAVPGTPPPDC
|||.|.||||.| | |.|||||||||.||.|||..|...                 |....  ||..|.||.|.|||||.|||||..|.. .. ......
FEEIQCLGKGGFGGQVYKVQNKLDGCYYAIKRIKLNWKQRKQPCITQECMIHKCKKHCEKVLREVKTLARLNHPNIVRYHNAWIEKPEEKWQQQQDEMWQ

TPQAQDSPATC-------------------------------GKTSGDTEELGSVEAAAPPPILSSSVEWSTSAERSTST--------------------
.....|.|...                               ....|....  ..... .. ....|.|||.|.....|                     
LDSSTDWPNGKHKSKDYNFHPFEKEWDDEEAQESNVMSECEDREQKGKKKCWSESNSHSSKNNYEESMEWSCSEHSDSSSFPNIENQFSINFGTENENES

----RFPVTGQDSSSD------------------------------------------------------------EEDEDERDGVFSQSFLPASDSDS-
    ... . . || |                                                            .. .||......||....|.. | 
HISYWSSSVYMSSSWDNPFDSSFSNSCDSTNFNKDEDHSFFSCSSYSSCSTDCDDSKDSSPFNWDGGCDNWEQYEYSDQDDEHEQNDCQSKQCDSKNQSH

------DIIFDNEDEN--------SKSQNQDEDCNQKDGSHE---IEPSVTAEAVHYLYIQMEYCEKSTLRDTIDQG-----------------------
      ..|.|.||..        |.||.|.|..| .. ...    ..... . .||||||||||||.|||| ||..                       
KKCMEMTWIQDQEDNKNNCSHHIGSSSQSQEEMENNTNNNKNKFKQMHNTKQYQCHYLYIQMEYCEKKTLRDWIDNNGCPVQFNYWRRNSCKNHYNNYIM

---------------------LFRDTSRLWRLFREILDGLAYIHEKGMIHRDLKPVNIFLDSDDH-----VKIGDFGLAT-----------------DHL
                     .|.|. ..||.||.||.||.|||..|||||||||.|||||.|..     ||||||||||                 ...
NQCCFQSTWSMNMRQWDENNCMFWDKDQCWRIFRQILEGLNYIHSQGMIHRDLKPSNIFLDCDGQHHQMQVKIGDFGLATNHEMLSQSQKNYNQQDQSYW

AFTAEGKQDDQAGDGVIKSDPSG---------------------HLTGMVGTALYVSPEVQ---GSTKSAYNQKVDLFSLGIIFFEMSYHPMVTASERIF
...|.......  ..........                     |.||.|||.||.|||..   ...||.||.|||..|||||||||.|... | .||. 
KYVANDNCQWWNTNQQNSPTGQDTHMWSNNYHIQPTSMFYCEHCHHTGGVGTCLYMSPEQLSGQHYQKSHYNHKVDMYSLGIIFFEMWYPFFTTMMERVK

VLNQLRDPTSPKFPDDFD----DGEHTKQKSVISWLLNHDPAKRPTAMELLKSELL
.|..||| .|..||.||.    | ...||.. |.|.|.|||..||.|...|..|..
TLTDLRD-ESIQFPGDFPPKWFDNKYPKQADFIQWMLSHDPQQRPSAQQILQHEFF
Kinase GCN2.AA STE 589-1000 16 3.3e-55 189.75 In-house 1-359 (359) Show / Hide 
Range on Protein: 589-1000
Range on HMM: 1-359/359
Sequence Identity: 16% (87 aa)

FEELQLLGKGAFGAVIKVQNKL---------------DGCCYAVKRIPINPAS----------------RHFRRIKGEVTLLSRLH--HENIVRYYNAWI
.| .. .|||||| | |  |.                 |. .|.|...... .                  . .|. |. ......  |.|||.|| . .
WEGIEKIGKGAFGTVYKAMNHKNCNIHKTGEVENNGETGEIMAIKQMNLENCNNKDENQENQELENKNHEEMQQILKEIKIMKKCNHMHPNIVKYYGCYF

ERHERPAVPGTPPPDCTPQAQDSPATCGKTSGDTEELGSVEAAAPPPILSSSVEWSTSAERSTSTRFPVTGQDSSSDEEDEDERDGVFSQSFLPASDSDS
  ..                                                                                                
KEGHN-----------------------------------------------------------------------------------------------

DIIFDNEDENSKSQNQDEDCNQKDGSHEIEPSVTAEAV-HYLYIQMEYCEKSTLRDTID---QGLFRDT-----------SRLWRLFREILDG-LAYIHE
                                   .   ..|.| |||..  .|.| |.     .....           | . .  |.|| | |.|.|.
-----------------------------------HKNDDELWICMEYMDGGSLDDIIKHKYGCGKPLNHNRQHQHRFPESQIAYICRQILKGLLYYLHS

KGMIHRDLKPVNIFLDSDDHVKIGDFGLAT---DHLAFTAEGKQDDQAGDGVIKSDPSG----------HLTGMVGTALYVSPEV--QGSTK--SAYNQK
.. ||||.|| ||.|     ||. |||...   |..|   . .              ..          . . .|||  . .|||  |....  . |. |
HHIIHRDIKPANILL-----VKLCDFGVCGQLTDSMANI-Q-S--------------NNIIDCIETMQQKRNTFVGTP-WMAPEVIQQNGQGRDKGYDTK

VDLFSLGIIF-------FEMSY-HPM--VTASERIF-------VLNQLRDPTSPKFPDDFDDGEH---------------TKQKSVISWLLNHDPAKRPT
.|  |||.          ...  |||    .  . .       .| .  ....|..||.....|                 . |  |.  |. || ||||
CDIWSLGCTIIEMATGKPPWHDMHPMRAMFMIPFNYHMQQQFEQL-CIVNGPPPPLPDHLSQNEMISYGGSGTTYPHKWSPEFKDFINKCLQKDPNKRPT

AMELLK--SELL
| .|||   . .
ASQLLKSEHPFI
Kinase GCN2.AA S_TKc 589-1000 17 6.9e-47 163.01 SMART 1-231 (231) Show / Hide 
Range on Protein: 589-1000
Range on HMM: 1-231/231
Sequence Identity: 17% (77 aa)

FEELQLLGKGAFGAVIKVQNKLDGCCYAVKRIPINPASRHFRRIKGEVTLLSRLHHENIVRYYNAWIERHERPAVPGTPPPDCTPQAQDSPATCGKTSGD
.| |...|||||| | |...| .|  .|.|.|.                                                                   
YEILRKIGKGAFGKVYKCRHKKTGRIVAIKIIK-------------------------------------------------------------------

TEELGSVEAAAPPPILSSSVEWSTSAERSTSTRFPVTGQDSSSDEEDEDERDGVFSQSFLPASDSDSDIIFDNEDENSKSQNQDEDCNQKDGSHEIEPSV
                                                                       . . | .  ..      . .    .  . 
----------------------------------------------------------------------EHIRREIQILKK------HHPNIVKLYDVF

TAEAVHYLYIQMEYCEK--STLRDTIDQGLF------RDTSRLWRLFREILDGLAYIHEKGMIHRDLKPVNIFLDSDDHVKIGDFGLATDHLAFTAEGKQ
     ..||. ||||.    .|.| |..          .  . ......|...|.|.|..| ||||||| ||.||.  |.||.|||||.           
QD---DHLYMVMEYCDGDLGDLFDYIKKRGRHGLRFPFPE-HARFYMYQICCALEYCHSHGIIHRDLKPENILLDE--HIKICDFGLARQL---------

DDQAGDGVIKSDPSGHLTGMVGTALYVSPEVQGSTKSAYNQ-KVDLFSLGIIFFEMSY-HPMVTASERIFVLNQLRDPTSPKFPDDFDDGEHTKQKSVIS
                 | ..||  |..|||       |.  |.|  |.|.|..|| .  |     .   ......                   |. |.
-----------------TTFCGTPWYMAPEVL-----GYGKCKCDWWSCGCILYEMLCGYPPFP--QMQMMFKKIG---------------SPEAKDFIR

WLLNHDPAKRPTAMELLK-------SELL
 .|. ||.||||| |.|.        . .
KCLQKDPEKRPTA-EALQDEWDIKCHPWF
Kinase GCN2.AA Pkinase 795-1000 26 3.3e-40 143.27 Pfam 74-293 (293) Show / Hide 
Range on Protein: 795-1000
Range on HMM: 74-293/293
Sequence Identity: 26% (65 aa)

YLYIQMEYCEKS----TLRDTID---QGLF-RDTSRLWRLFREILDGLAYIHEKGMIHRDLKPVNIFLDSDDHV----KIGDFGLATDHLAFTAEGKQDD
..|. |||||.      | | |    .| |  .   . . ...|| ||.|.|..| ||||||| ||..|...|.    ||.|||||  .           
HIYMVMEYCEGGLYVMDLFDYISTWRHGCFYFSEWECKFYMYQILRGLEYCHSMGIIHRDLKPENILIDNNGHIDACVKICDFGLAKQFD----------

QAGDGVIKSDPSGHLTGMVGTALYVSPEVQGSTKSAYNQKVDLFSLGIIFFEMSYHPMVTASE------RIFVLNQLRDPTSPKFPDDFDDGEH------
          ... .|. .||  |..|||   . . | .||| .|.|.|..||        ..      .  .. ... .. ..|. . .. .       
--------Y-NNS-MTTFCGTPWYMAPEVIR-GGQYYGPKVDMWSCGCILYEMLCGRPPFPGHCWHDNHDQMQMICRIMGPPLHFDWPWWCSISYFFFRH

-----------TKQKSVISWLLNHDPAKRPTAMELLKSELL
              |..|.|.|. ||.|||||...|... .
WHFHWTFHNWSEECKDFIKWCLCKDPKKRPTAEQILQHPWF
Kinase GCN2.AA CAMK 594-931 13 2.3e-37 117.44 In-house 1-323 (425) Show / Hide 
Range on Protein: 594-931
Range on HMM: 1-323/425
Sequence Identity: 13% (63 aa)

LLGKGAFGAVIKVQNKLDG------------CCYAVKRIPINPASRH---------------------------FRRIKGEVTLLSR-------LHHENI
 ||.|.|| | ....|...              .|.| |. . .. .                            .... |. ....       |.|.||
TLGRGSFGKVKLCIHKNTTGKERVHHIYTNGQKVAIKIIDKKKLKDKQNQDYSNCYKIDKSKCKHRYCWRRPKDRKNVRREIEIMKKLQGIGNKLDHPNI

VRYYNAWIERHERPAVPGTPPPDCTPQAQDSPATCGKTSGDTEELGSVEAAAPPPILSSSVEWSTSAERSTSTRFPVTGQDSSSDEEDEDERDGVFSQSF
.. | ..  .|  .    |                                                                                 
IKLYEVYEDPHYFK-N--T---------------------------------------------------------------------------------

LPASDSDSDIIFDNEDENSKSQNQDEDCNQKDGSHEIEPSVTAEAVHY------------LYIQM---EYCEKS----TLRDTIDQG-----------LF
                                               |            .|. |   |||.       | | | .            . 
--------------------------------------------ND-YETSKQVFTKDKHMYMVMEIMEYCSGGELFDDLFDYIVEHGCYGEEFDQFDIV

RDTSRL-----WRLFREILDGLAYIHEKGMIHRDLKPVNIFLDSDD-----------------HVKIGDFGLATDHLA---------FTAEGKQDDQAGD
 .. |.      |.|| |.....|.|... .|||||| || ||...                  .|| |||.|.             .  .  . ...  
-ERGRFTEREARRYFRQICSAVHYCHSHNIVHRDLKPENILLDDNNDDSDPVEHDEIFDWNNNNIKIIDFGFANKFDPGENNFMCSDYKGGE-M-HNQ--

GVIKSDPSGHLTGMVGTALYVS-PEVQGS-T-----------KSAYNQKVDLFSLGII
 .  ...   .. ..||  |.. |||  . .           |  |..|.|  |.|.|
-WTTPTEGQKMKTFCGTPEYMAAPEVLNGKGHCNMQNATDEEKPYYGPKCDMWSCGVI
Kinase GCN2.AA STE-Unique 794-1000 26 3.1e-36 125.95 In-house 367-616 (616) Show / Hide 
Range on Protein: 794-1000
Range on HMM: 367-616/616
Sequence Identity: 26% (66 aa)

HYLYIQMEYCEKSTLRDTIDQ-GL---------FRDTSRLWRLFREILDGLAYIHEKG--MIHRDLKPVNIFLDSDD-----HVKIGDFGLATDHLAFTA
. ..|.|||||..||.. |.. |          |. | .  .. ..|| || |.| ..   ||||||| |||...|.     .|||.|||.||   .   
NWVWIFMEYCEHGTLQQIIKRCGCPGNPNNLQLFPET-EVIWYTYQILEGLEYLHSQHNPIIHRDLKPHNIFCMQDGKGRVCKVKICDFGHATCCQPDHF

EGKQDDQAGDGVIKSDPS-------------------GHLTGMVGTALYVSPEVQGST---KSAYNQKVDLFSLGIIFFEM-SYHPMVTASERIFVLNQL
|     .     | .  .                     .....||  | .|||        . |. ||| .|||...|.| . .|  .   ..  . ..
EKDNNSKNSNTWININNNSSTNSTAQQTPMWDLNINNYTMKYCCGTPCYMCPEVINCRNQINNPYDTKVDIWSLGCMCFHMCCGCPPWHQRYHMQAYIEM

RDPTSPKFP----DDFDDGEHTKQKSVISWLLNHDPAKRPTAMELLKSELL
...     |    ..  .     |   |  .|. .| .||.|..||....|
CIHIHCQPPQCPIENIPPKCQPEQQQFIEKCLERNPHHRPSAHQLLHHFWL
Kinase GCN2.AA Ciliate-E2 794-1000 22 9.1e-35 116.76 In-house 107-365 (365) Show / Hide 
Range on Protein: 794-1000
Range on HMM: 107-365/365
Sequence Identity: 22% (60 aa)

HYLYIQMEYCEKSTLRDTIDQG-LFR----------DTSRLWRLFREILD---------------GLAYIHEKG-MIHRDLKPVNIFLDSDDHVKIGDFG
...|. ||||.  .|.| | .  ..|          .     .....||.               .| |.|.|.  ||||.|| ||..     .|| |||
NNIYLVMEYCQGGDLFDYIKNNKKKRNHEIEKDKYFTEKEIKYIMKQILQGFKTKAEFYIACIILALEYLHSKNNIIHRDIKPENILF-----IKIIDFG

LATDHLAFTAEGKQDDQAGDGVIKSD--PSGHLTGM------------VGTALYVSPEVQGSTKSA-------YNQKVDLFSLGIIFFEMSYH--PMVTA
|...   .  .. .  |     .      |.. . |            .||. |..||. .. |         |. |.|..|||.|..|| .   |    
LSKKYDDDNQNRTH-KQQCKWYMF-KNDTSRTPGYMDQIIQQNMFNTICGTPGYMAPEILNGKKYDNQQKQKGYDYKCDIWSLGVILYEMLFGKPPF---

SERIFVLNQLRDPTSPKFPDDFDD---------GEHTKQ-KSVISW----LLNHDPAKRPT-AMELLKSELL
   .  ...  |  .|.. .| ..          ..... |  |.     .|. || ||.| . ..|. . .
--QQQIKQCKIDFPCPEWQKD-TQCIQCKWKSWKKWSQECKDLIKKFGKRMLQKDPEKRITGWEQILNHPWF
Kinase GCN2.AA PEK 795-875 38 2.4e-29 94.2 In-house 413-520 (758) Show / Hide 
Range on Protein: 795-875
Range on HMM: 413-520/758
Sequence Identity: 38% (42 aa)

YLYIQMEYCEKSTLRDTIDQG---LFRDTSRLWRLFREILDGLAYIHEKGMIHRDLKPVNIFLDSDDH------------------------VKIGDFGL
|.||||..|.| ||.| .        |. .    .|  |..|. ||| |||||||||| |||. .|.                         .|||||||
YMYIQMQLCRKETLKDWMRNRCTMESRPWMHMLHWFHQICEGVNYIHSKGMIHRDLKPSNIFFSMDSQHHQQSSFSNISSSFEENSDGGHQIIKIGDFGL

ATDHLAFT
.||| . |
VTDHTIVT
Kinase GCN2.AA PEK 898-1000 32 1.4e-25 81.35 In-house 646-758 (758) Show / Hide 
Range on Protein: 898-1000
Range on HMM: 646-758/758
Sequence Identity: 32% (38 aa)

TGMVGTALYVSPE-VQGS-------------TKSAYNQKVDLFSLGIIFFEMSYHP-MVTASERIFVLNQLRDPTSPKFPDDFDDGEHTKQKSVISWLLN
|. ||| || ||| . .               |  || ||| .|||.||||. .||   |..||| ..  ||.    ..| .|.. .. ..   |.... 
TVQVGTHLYMSPEQCHHQSVFGTGTYTNPEQKKTWYNHKVDIYSLGLIFFEL-IHPGFSTQMERIRTMCDLRN---GQWPKWFTQ-KYPQEYEFIQQMMS

HDPAKRPTAMELLKSELL
..|  |||| ..... . 
MNPDERPTADQISMHDFF
Kinase GCN2.AA WEE 796-870 32 1.5e-23 75.94 In-house 80-181 (297) Show / Hide 
Range on Protein: 796-870
Range on HMM: 80-181/297
Sequence Identity: 32% (33 aa)

LYIQMEYCEKST--LRDTIDQGLFRDT----SRLWRLFREILDGLAYIHEK-GMIHRDLKPVNIFLDSDD--------------------HVKIGDFGLA
.|||||||...   | . | | .  .     ...| ....|. ||..||.. ||.| |.|| |||. ...                      ||||||..
MYIQMEYCNGGSNLLQFWIQQCHNWNHWLPEWMIWQILVDICQGLHHIHSNCGMVHMDIKPENIFISKKMNCNQIVNNDQQNNNDWWFNMMCKIGDFGHV

TD
||
TD
Kinase GCN2.AA WEE 589-656 39 5.3e-23 74.06 In-house 1-76 (297) Show / Hide 
Range on Protein: 589-656
Range on HMM: 1-76/297
Sequence Identity: 39% (30 aa)

FEELQLLGKGAFGAVIKVQNKLDGCCYAVKR--IPINPAS---RHFRRIKGEVTLL---SRLHHENIVRYYNAWIE
|.|.| .|.| || | |.....||| ||.|.   |..  |   |..| ... . |    .  ||..|||||.||.|
FQEVQKIGEGSFGEVYKCRCRIDGCIYAIKKSKQPFRGYSERKRALREVYAHMVLGQIMDCDHHPHIVRYYSAWEE
Kinase GCN2.AA PEK 589-657 40 2.2e-22 70.53 In-house 1-77 (758) Show / Hide 
Range on Protein: 589-657
Range on HMM: 1-77/758
Sequence Identity: 40% (31 aa)

FEELQLLGKGAFGAVIKVQNKLDGCCYAVKRIPINP--------ASRHFRRIKGEVTLLSRLHHENIVRYYNAWIER
||. | ||.| || | ...||.|.. ||.|||.           ..    .   ||  |  | | |||||.||| |.
FEHIQCLGHGGFGVVFRAKNKVDMNNYAIKRITFPNSNNQSPTNRESAREKVMREVRTLAMLDHHNIVRYFNAWLET
Kinase GCN2.AA STE11 828-911 37 3.9e-16 55.09 In-house 123-206 (303) Show / Hide 
Range on Protein: 828-911
Range on HMM: 123-206/303
Sequence Identity: 37% (32 aa)

REILDGLAYIHEKGMIHRDLKPVNIFLDSDDH-VKIGDFGLATDHLAFTAEGKQDDQAGDGVIKSDPSGHLT-GMVGTALYVSPEV
. || ||.| |..| ||||.|. ||.|||| . .|..||| |. . . . .......  .  .||.. | .  .|.||. . .|||
KQILEGLQYLHSHGIIHRDIKGANILLDSDGNVCKLADFGCAKRLAWICTCTHNMNMTQN-HCKSMT-GTPYNSMMGTPMWMAPEV
Kinase GCN2.AA STE-Unique 590-656 33 2.5e-15 50.42 In-house 1-72 (616) Show / Hide 
Range on Protein: 590-656
Range on HMM: 1-72/616
Sequence Identity: 33% (24 aa)

EELQLLGKGAFGAVIKVQNKLDGCCYAVKRIPINPASRH-----FRRIKGEVTLLSRLHHENIVRYYNAWIE
.. | .|.|.|| | . ||| |.  .|.| |...  .          |  |......|||.|||.|| .|..
NCQQMIGRGSFGNVHNCQNKKDCFRCAIKVIHLKQFKDFNQEPLVNMIISEIEIMKCLHHQNIVKYYGHWLW
Kinase GCN2.AA STE20 828-935 36 9.2e-15 50.64 In-house 124-220 (288) Show / Hide 
Range on Protein: 828-935
Range on HMM: 124-220/288
Sequence Identity: 36% (40 aa)

REILDGLAYIHEKGMIHRDLKPVNIFLDSDDHVKIGDFGLATDHLAFTAEGKQDDQAGDGVIKSDPSGHLTGMVGTALYVSPEV-QGST--KSAYNQKVD
||.| ||.|.|....||||.|  || |..|  ||. |||.. .   .| .|   | . ..  ..        .|||  . .||| |. .  .. |..|.|
REVLQGLDYLHSNHIIHRDIKADNILLTEDGEVKLADFGVCAQ---LTKHG-R-D-TMQFKRNT--------FVGTPYWMAPEVIQCMEGPDKGYDYKCD

LFSLGIIFFEM
  ||||   ||
IWSLGITAIEM
Kinase GCN2.AA STE20 589-659 24 1e-14 50.49 In-house 1-82 (288) Show / Hide 
Range on Protein: 589-659
Range on HMM: 1-82/288
Sequence Identity: 24% (20 aa)

FEELQLLGKGAFGAVIKVQNKLDGCC----YAVKRIPINPAS---RHFRRIKGEVTLLSRLHHENIVRYYNAWIER----HE
.| |. .|.|..|.| |. .|  | .    .|.|........    ... |. |.  .....|.|||.||.. .      .|
YELLEKIGQGSYGKVYKAYHKPTGEYCNQRVAIKKMNLEKCDNEQDEWEDIQKEISIMKQCKHPNIVKYYGSYLKDDTCDDE
Kinase GCN2.AA STE11 589-651 34 6.4e-14 46.98 In-house 1-76 (303) Show / Hide 
Range on Protein: 589-651
Range on HMM: 1-76/303
Sequence Identity: 34% (26 aa)

FEELQLLGKGAFGAVIKVQNKLDGCCYAVKRIPINPA-------------SRHFRRIKGEVTLLSRLHHENIVRYY
. . ...|||||| | |  |.  |  .||| |.|..               . .. |. |. |.. |.|.||||||
WQKGEIIGKGAFGTVYKCMNVDTGELMAVKQIEIQNNNQMKEDQEKEYQSHKMHKAIQEEIKLMKHLNHKNIVRYY
Kinase GCN2.AA Pkinase 589-656 29 1.5e-13 49.04 Pfam 1-71 (293) Show / Hide 
Range on Protein: 589-656
Range on HMM: 1-71/293
Sequence Identity: 29% (21 aa)

FEELQLLGKGAFGAVIKVQNKLDGCCYAVKRIPINPASRHFRRIK---GEVTLLSRLHHENIVRYYNAWIE
.. .. .|.|.|| | | ..| .|. .|||.|.   ..  .....    |. .. ||.|.||||.| .. .
YHIGRKIGSGSFGCVYKCHHKGTGKIVAVKIIKKRHEKSWKDQTHRRLSEIQIMKRLNHPNIVRLYDWFED
Kinase GCN2.AA TyrKc 589-1000 14 9.5e-11 -48.89 SMART 1-280 (280) Show / Hide 
Range on Protein: 589-1000
Range on HMM: 1-280/280
Sequence Identity: 14% (66 aa)

FEELQLLGKGAFGAVIKVQNKLDGCCYAVKRIPINPASRHFRRIKGEVTLLSRL--HHENIVRYYNAWIE---------------------------RHE
 . .. || |||| | | . |      ||| .  .      . .  |  ....|  ||.|||| | . ..                           |  
ITLGKKLGEGAFGEVYKGTWK---IQVAVKMLKEDA----REDFMREARIMCKLGGHHPNIVRLYGVCTQQGRNFMYIEPLMIVMEYMPHGDLKDYLRKH

RPAVPGTPPPDCTPQAQDSPATCGKTSGDTEELGSVEAAAPPPILSSSVEWSTSAERSTSTRFPVTGQDSSSDEEDEDERDGVFSQSFLPASDSDSDIIF
||                                                                                                  
RPK-------------------------------------------------------------------------------------------------

DNEDENSKSQNQDEDCNQKDGSHEIEPSVTAEAVHYLYIQMEYCEKSTLRDTIDQGLFRDTSRLWRLFREILDGLAYIHEKGMIHRDLKPVNIFLDSDDH
                                                            .. |      | .| .|.|.|. |||||   |. .... .
----------------------------------------------------------LCMKDLLSFCWQIACGMEYLHSKNCIHRDLAARNCLVGENHT

VKIGDFGLATDHLAFTAEGKQDDQAGDGVIKSDPSGHLTGMVGTALYVSPEVQGSTKSAYNQKVDLFSLGIIFFEM------SYHPM----VTASERIFV
|||.||||. |.    ..|     . . ....  ...  .|.      .||.    .. .. | | .| |.  .|.       |  |    ....|   |
VKISDFGLSRDIYDDDQQG-----ESKDYYRKKEHKGGKTMLPIR-WMAPECI--NYGKFTTKSDVWSFGVCMWEIFTYGQQPYPGMIQQMMSNQE---V

LNQLRDPTSPKFPDD-----FDDGEHTKQKSVISWLLNHDPAKRPTAMELLKSELL
......   .. |..     |  . ...  .....  ..||..|||  |.   |..
IEYVKQGYRMPQPPNDYPMSF-VCCPDEMYQIMKQCWHEDPEDRPTFSEI--HECF
Kinase GCN2.AA Ciliate-E2 589-656 19 2.7e-09 28.03 In-house 1-103 (365) Show / Hide 
Range on Protein: 589-656
Range on HMM: 1-103/365
Sequence Identity: 19% (20 aa)

FEELQLLGKGAFGAVIKVQNKLDGC-----------------------CYAVKRIP------INPASRHFRRIK------GEVTLLSRLHHENIVRYYNA
.. ....|.|.|| | |........                        ||.|..       |.   . .|.|.       |  .|..| | ||...| .
YKFIKKIGQGNFGSVYKCKVWKVQKQFAIKIIDQKQQRQQMQEKVKIKIYAMKIMKKNQIEQIKQEIHIMREINCFYKKINEKNILEQLNHPNIIKMYEC

WIE
..|
FQE
Kinase GCN2.AA Pkinase_Tyr 832-871 45 1.2e-08 29.96 Pfam 122-161 (270) Show / Hide 
Range on Protein: 832-871
Range on HMM: 122-161/270
Sequence Identity: 45% (18 aa)

DGLAYIHEKGMIHRDLKPVNIFLDSDDHVKIGDFGLATDH
.|..| ..|..|||||   |. .... .|||.||||| |.
KGMEYLESKNCIHRDLAARNCLVTENNVVKICDFGLARDI
Kinase GCN2.AA WEE 974-1000 40 8.5e-08 22.31 In-house 271-297 (297) Show / Hide 
Range on Protein: 974-1000
Range on HMM: 271-297/297
Sequence Identity: 40% (11 aa)

KSVISWLLNHDPAKRPTAMELLKSELL
|..| | .. || .|||...||....|
KQLIKWMMHPDPEQRPTCQQLLQHPVL
Kinase GCN2.AA CAMK 974-1000 33 2e-06 16.3 In-house 399-425 (425) Show / Hide 
Range on Protein: 974-1000
Range on HMM: 399-425/425
Sequence Identity: 33% (9 aa)

KSVISWLLNHDPAKRPTAMELLKSELL
|. |...|. || ||.|. . |. . .
KDLIRKMLQVDPEKRMTIEQCLNHPWM
Kinase GCN2.AA Pkinase_Tyr 589-651 27 0.000158 15.96 Pfam 1-70 (270) Show / Hide 
Range on Protein: 589-651
Range on HMM: 1-70/270
Sequence Identity: 27% (21 aa)

FEELQLLGKGAFGAVIKVQNKLDGCCY----------AVKRIPINPASRH--FRRIKGEVTLLSRL-HHENIVRYY
 . .. ||.|||| | |      | ..          ||| .. .  ...   .    |. ... | ||.||||.|
ITFGKKLGEGAFGEVYK------GTWKGDPDNTKIPVAVKMLKEGAMHEQNCREDFMREAKIMKQLGHHPNIVRLY
Kinase GCN2.AA STE20 977-1000 45 0.000198 12.44 In-house 265-288 (288) Show / Hide 
Range on Protein: 977-1000
Range on HMM: 265-288/288
Sequence Identity: 45% (11 aa)

ISWLLNHDPAKRPTAMELLKSELL
..  |. ||.||||| .||| . .
VDKCLQKDPEKRPTASQLLKHPFI
Kinase GCN2.AA STE11 981-1000 50 0.000938 9.79 In-house 284-303 (303) Show / Hide 
Range on Protein: 981-1000
Range on HMM: 284-303/303
Sequence Identity: 50% (10 aa)

LNHDPAKRPTAMELLKSELL
. ||| |||||..||.   .
FEHDPKKRPTANQLLQHPFI