Gene W09C3.1 (Nematode worm)

W09C3.1

Species: Nematode worm
Alias: CE14744, aSWK099, W09C3.1
External Links: Wormbase Link ,
Annotation: WormBase Genetic Map location: I:-0.69, WormBase Physical Map location: I: 4717912-4719576

Classification

Group: CK1
Family: TTBKL

Sequence

Name Sequence Type Origin Length Description Download
W09C3.1.kin_dom Protein Kinase Domain Sugen global kinase HMM prediction 256 Kinase domain by global Sugen HMM Fasta, JSON
W09C3.1.AA Protein Genbank 429 None Fasta, JSON

Protein domain

Protein domains of W09C3.1.AA. The domains are annotated by HMM profiles from Pfam and SMART, as well as in-house data which includes HMMs of each individual kinase group, family and subfamily. Here is domain list in details, including sequence identity, significance and alignment. In particular, you can find can find the best hit kinase group/family/subfamily, which is helpful to understand the relationship between kinases.Kinase domain and best hit kinase group/family/subfamily are highlighted in red and blue. Visualized by pviz.

Usage: Zoom in selected region by dragging mouse and zoom out by double-clicking.

Show/hide domain list
Domain Protein name Domain Name Range Identity (%) Significance Score Profile Source Profile Range (length) Alignment
Kinase W09C3.1.AA TTBKL 23-284 41 8.9e-143 480.62 In-house 1-288 (288) Show / Hide 
Range on Protein: 23-284
Range on HMM: 1-288/288
Sequence Identity: 41% (120 aa)

WLTIRELGRGAFGTVYHVSNKST-KQEAALKIEKMT-AG-DNLLKFEREIMVAMQ--HEPTAIHIYDDGIYKDYRYIVMTLCGPDLQKIAELMNNN--FN
|. ....|.|.||.||||..... |..||.|.||.. .| |. .|.| ......|  . | ...... | .|||...|||||||.||..   |.|.  |.
WMIVKMIGEGGFGQVYHVWDMKDKKDCAAMKEEKNRLEGGDSRMKMEVHVLMKLQPNGSPHFPKCHCSGKRKDYNFMVMTLCGPNLQDLRKMMPNKKRFS

QDTIIRVCIRTLLAIKTMHEYTYIHRDLKPCNFAVDFN----PNSLHVYLFDYGMARKYATKDSGN-------KWQLRRPRN---PAQFRGTVRYCSLNM
. |. |..|..| |.| ||.. .||||.||||......    ... ||||.|.||||.||.||...       ||..|||||    |.||||.||||.||
DGTWSRIGIQCLYALKQMHDCGFIHRDVKPCNCCWGYHDHSCERCRHVYLIDFGMARQYAWKDGEWNGPIHENKWMMRRPRNFRHKAPFRGTTRYCSPNM

HKRKELSRVDDLWSWFFMLMEMYAPLPWTSSNIPERIEALKEDHLNEYLSKDS-----FLSSFLPLVEYLNSVQYADRPDYMKIYDIL
| |||..|.||||||..|..|....||||... ...||..|.... |.|.||.     . |.|.. ...| | .|.|||||..|| ..
HDRKEQGRHDDLWSWMYMIIELHCGLPWTNMEDKMEIEHMKKNMKDEDLFKDMPRDHDCPSEFCKFMKHLRSLRYYDRPDYEMIYECF
Kinase W09C3.1.AA CK1 23-280 29 1e-105 357.55 In-house 1-339 (339) Show / Hide 
Range on Protein: 23-280
Range on HMM: 1-339/339
Sequence Identity: 29% (101 aa)

WLTIRELGRGAFG-TVYHV-----SNKSTK-----QEAALKIEKMTAG--DNLLKFEREIMVAMQH--------------------EPTAIHIYDDGIYK
|  .. .| |.|| ..|.|     ......     .|.|.|.|. ..   . .|. | ... ..|.                    .|  ..... |  .
WKVVKKIGEGGFGYQIYKVQKEDHKDMKNNKPYKGEEYAMKVEPKKSMGQHPQLMEEYQMYKKLQGGKKWKASILKHKHFCKKHCGCPHPCMYWYHGEED

DYR-YIVMTLCGPDLQKIAE--LMNNN-FNQDTIIRVCIRTLLAIKTMHEYTYIHRDLKPCNFAVDF-------------NPNSLHVYLFDYGMAR-KYA
||  |.||||.||.|... .   |.|. |.  |.... |..| |.. ||.  .||||.||.||...              |... .||..|.|||| ||.
DYYNYMVMTLLGPNLEDLRKQNRMCNRKFSMKTWLMIAIQMLEALEYMHDCGFIHRDIKPDNFCMGRNESFKYGKHHHEWNNEHRQVYMIDFGMARRKYR

TKDSGN--------KWQLRRPRN---PAQFRG-TVRYCSLNMHKRKELSRVDDLWSWFFMLMEMYAP---LPWTSSNIPER-----IEALKEDHLNEYLS
.|| .|        .||.|.||.    ..||| |.||||.|.|..||..|.|||||||.||.|.  .   |||.  . ...     .|  |... .| . 
DKDGHNRWNGPRDKTWQHRPPREHKKKKGFRGCTARYCSINCHLGKEQGRRDDLWSWFYMLIEWLKGTPGLPWQNMKDEMKPKDHQVEKMKYEFIDEKKM

KDSFL-----------SSFLPLVEYLNSVQYADRPDYMKI
...|.           ..| ....|..| .| |||||..|
STPFFLCCPKKDHDCPQEF-KIMKYIRSLKYYDRPDYEMI
Kinase W09C3.1.AA Worm6 23-280 32 7.1e-62 211.87 In-house 1-279 (279) Show / Hide 
Range on Protein: 23-280
Range on HMM: 1-279/279
Sequence Identity: 32% (92 aa)

WLTIRELGRGAFGTVYHVSNKSTKQ--EAALKIEK--MTAGDNLLKFEREIMVAMQHEP--TAIHIYDDGIYKD--YRYIVMTLCGPDLQKI-AELMNNN
|   ...| |.||.|| |..||.|   . |.| ||   ..  . ||.|  .. |  |..      | | | ..   ..||||||.|..|. . ...... 
WKVTKKIGEGGFGAVYKVEDKSDKKKKQFAMKCEKQMENMKRQMLKMECMVLKAVKHHKCRHFCKIEDRGKVDNRGFNYIVMTLVGKNLWDLRKNRPDRK

FNQDTIIRVCIRTLLAIKTMHEYTYIHRDLKPCNFAVDF-----NPNSLHVYLFDYGMARKYATKDSGNKWQLRRPR-NPAQFRGTVRYCSLNMHKRKEL
|   | ... | .| ||  .|   .||||.|| ||..       . ..  .|..|.||.|...... ||  .|| || ....|||||||..|. ||..|.
FSMGTGLKIAIQCLEAIEDLHNCGFIHRDVKPGNFCIGRYQMKEDNEHHTIYMLDFGMCRRKKYCNKGN--ELRTPRWTACGFRGTVRYAPLACHKGQEQ

SRVDDLWSWFFMLMEMYAP-LPWTSSNIPERIEALKED---HLNEYLSKDSFL---S-SFLPLVEYLNSVQYADRPDYMKI
.| ||. ||| |. ||  . |||  .. . . | .|.    .. ... .. |       |    .|. . .| ||||| .|
CRKDDCESWFYMMVEMIVGCLPWRHMKDHDHVEKHKKWCRRRTRAPMMRMFFGPCPKEEFNDIMDYIDQYSYWDRPDYKFI
Kinase W09C3.1.AA TTBK 23-280 32 2.2e-48 167.04 In-house 1-257 (257) Show / Hide 
Range on Protein: 23-280
Range on HMM: 1-257/257
Sequence Identity: 32% (85 aa)

WLTIRELGRGAFGTVYHVSNKSTK-QEAALKIEKMTAGDNLLKFEREIMVAMQHEPTAIHIYDDGIYKDYRYIVMTLCGPDLQKI-AELMNNNFNQDTII
|  ..  | |.|| .|   .  ..  . |.|.|.  .    || |  ..  .|  . .   .  |   .. |.||.| |  |     .     |.  | .
WKVVKKIGGGGFGEIYEAKDMINHHEQVAIKVESAQQPKQVLKMEVAVLKKLQGCDHVCRFIGCGRNDKFNYMVMQLQGKNLADLRRKQPRGCFSLSTTL

RVCIRTLLAIKTMHEYTYIHRDLKPCNFAVDFNPNSL-HVYLFDYGMARKYATKDSGNKWQLRRPRNPAQFRGTVRYCSLNMHKRKELSRVDDLWSWFFM
|. .  | .|.  |.  . ||| || |||.  ||     .|. |.|.||.| |  .|   . | ||. | ||||||| | | |. .|. | ||||| |.|
RLGVQILQSIESIHSVGFLHRDIKPSNFAMGRNPSTCRKCYMLDFGLARQY-TNACG---EVRPPREVAGFRGTVRYASINAHRNREMGRHDDLWSLFYM

LMEMYA-PLPWTSSNIPERIEALKEDHLNEYLSKDSFLSSFLPLVEYLNSVQYADRPDYMKI
| |     |||      . .  .||      | |.   . |   .. ..| .|.|||||  |
LVEFVQGQLPWRKIKDKDQVGEMKEKYDHRMLLKH-CPHEFHQFMDHIKSLNYEDRPDYQLI
Kinase W09C3.1.AA CK1-A 23-280 29 9.3e-31 105.84 In-house 1-261 (261) Show / Hide 
Range on Protein: 23-280
Range on HMM: 1-261/261
Sequence Identity: 29% (78 aa)

WLTIRELGRGAFGTVYHVSNKSTKQEAALKIEKMTAGDNLLKFEREIMVAMQHEPTAIHIYDDGIYKDYRYIVMTLCGPDLQKIAELMNNNFNQDTIIRV
.  .|..| |.|| .|   |    .| |.|.|.  |    | .|  .   .|  .   |.   |  |||  .|| | || |  .  . .  |   |.. .
YRLVRKIGSGSFGDIYLGINITNGEEVAIKMESNKARHPQLIYEAKVYRILQGCVGIPHMRWYGCEKDYNVMVMDLLGPSLEDLFNFCKRRFTMKTVLML

CIRTLLAIKTMHEYTYIHRDLKPCNFAVDFNPNSLHVYLFDYGMARKYATKDSGNKWQLRRPRNPAQFRGTVRYCSLNMHKRKELSRVDDLWSWFFMLME
    .  |  .|| ..||||.|| || .  .    ...| |.|.|.||  . .    . |  .|     ||.|| |.| |   | || ||. |  ...| 
ADQMIMRIEYVHEKNFIHRDIKPDNFLMGIGRHCNKLFLIDFGLAKKYRDSRTRQHIPYREDKN---LTGTARYASINAHLGIEQSRRDDMESMGYVMMY

M-YAPLPW------TSSNIPERIEALKEDHLNEYLSKDSFLSSFLPLVEYLNSVQYADRPDYMKI
   . |||      |     |.|   |     | | |  | . |  ...|  .... . |||| .
FNRGKLPWQGMKAMTKKQKYEKISEKKMSTPIEQLCK-GFPAEFCMYMNYCRNLRFEEQPDYMYL
Kinase W09C3.1.AA CK1-Unclassified 23-213 31 3.1e-30 86.46 In-house 1-199 (244) Show / Hide 
Range on Protein: 23-213
Range on HMM: 1-199/244
Sequence Identity: 31% (63 aa)

WLTIRELGRGAFGTVYHVSNKSTKQEAALKIEKMTAGDNLLKFEREIMVAMQHEP-------TAIHIYDDGIY--KDYRYIVMTLCGPDLQKIAELMNNN
   |. .| |.|| .|.. |. |..|.|.|.|| ..   .|..|  || ..|..           ..|  |     || ..||.| ||.| ..  ..|  
YRVIEKIGQGSFGEIYKCINMQTNEEVAIKLEKKNSKHPQLCYEAKIMQYLQNGHNCDNFIKGIPKMYWCGQEEQGDYNIMVMDLLGPSLEDLFNMCNRK

FNQDTIIRVCIRTLLAIKTMHEYTYIHRDLKPCNFAVD-FNPNSLHVYLFDYGMARKYATK-DSGNKWQLRRPRNPAQFRGTVRYCSLNMHKRKELSRVD
|.  |.   .   .  |  .|.  .||||.|| || .   . |. .||. |.| . .| ..   |.  . |. .|   . || || |.|.|. .| || |
FSLKTVLMIADQMIQRIEYLHNKNFIHRDIKPDNFLMGKLGKNHHQVYIIDFGLSKRYIDMDKTGKHIPYRENKN---LTGTARYASINAHLGIEQSRRD

DL
||
DL
Kinase W09C3.1.AA CK1-D 23-280 30 1.2e-28 98.09 In-house 1-261 (261) Show / Hide 
Range on Protein: 23-280
Range on HMM: 1-261/261
Sequence Identity: 30% (80 aa)

WLTIRELGRGAFGTVYHVSNKSTKQEAALKIEKMTAGDNLLKFEREIMVAMQHEPTAIHIYDDGIYKDYRYIVMTLCGPDLQKIAELMNNNFNQDTIIRV
.   | .| |.|| .||  | .|  | |.|.| . .    |..|  .   ||      .|   |   ||  .|| | || |  .    .  |   |.. .
YRIGRKIGSGSFGDIYHGTNIQTNEEVAIKLECIKTKHPQLHIESKFYKMMQGGIGIPEIRWCGQEGDYNVMVMDLLGPSLEDLFNYCHRKFSLKTVLML

CIRTLLAIKTMHEYTYIHRDLKPCNFAVDFNPNSLHVYLFDYGMARKYATKDSGNKWQLRRPRNPAQFRGTVRYCSLNMHKRKELSRVDDLWSWFFMLME
    .  |. .|   .||||.|| || .        ||. |.|.|..|    .      |  .|     || || |.| |.  | || ||| |  ..|| 
ADQMICRIEYIHCRNFIHRDIKPDNFLMGLGKRGNMVYIIDFGLAKRYRDARTHQHIPYRENKN---LTGTARYASINTHQGIEQSRRDDLESLGYVLMY

MYA-PLPW------TSSNIPERIEALKEDHLNEYLSKDSFLSSFLPLVEYLNSVQYADRPDYMKI
     |||      |  .  |||   | .   . | |  . | |   ..|  .... |.|||. .
FNRGSLPWQGLKAATKRQKYERICEKKMSTKIDTLCK-GYPSEFQQYMNYCRNLKFDDKPDYCYL
Kinase W09C3.1.AA CK1 23-213 30 2.3e-26 87.89 In-house 1-199 (271) Show / Hide 
Range on Protein: 23-213
Range on HMM: 1-199/271
Sequence Identity: 30% (61 aa)

WLTIRELGRGAFGTVYHVSNKSTKQEA---ALKIEKMTAGDNLLKFEREIMVAMQH------EPTAIHIYDDGIY--KDYRYIVMTLCGPDLQKIAELMN
   .| .| |.|| .|.. |  |..|.   | |.||...   .|..|  |.  .|.      ..   |.|  |    .|| ..||.| ||.| .   ..|
YRVGRKIGQGSFGDIYLGINM-TNGEENHVAIKLEKNKSKHPQLHYEAKIYKILQGHNCDNQCHGIPHMYWCGQEEQGDYNIMVMDLLGPSLEDLFNFCN

NNFNQDTIIRVCIRTLLAIKTMHEYTYIHRDLKPCNFAVDF-NPNSLHVYLFDYGMARKYATKDSGNKWQLRRPRNPAQFRGTVRYCSLNMHKRKELSRV
  |   |.. ..   .  |  .|.  .||||.|| || .   . .. .||. |.|.| .|   ....  . |  .|   . ||.|| |.|.|  .| || 
RKFSLKTVLMIADQMIQRIEYVHNKNFIHRDIKPDNFLMGRYGKHCHQVYIIDFGLAKRYRDDKTHQHIPYRENKN---LTGTARYASINTHLGIEQSRR

DDL
||.
DDM
Kinase W09C3.1.AA CK1-Unique 23-221 25 1e-25 86.18 In-house 1-221 (337) Show / Hide 
Range on Protein: 23-221
Range on HMM: 1-221/337
Sequence Identity: 25% (58 aa)

WLTIRELGRGAFGTVYHVSNKSTK------QEAALKIEKMTAGDN--LLKFEREIMVAMQHEPTAIH-------IYDDGIYKDYRYIVMTLCGPDLQKIA
|  | ..| | .|.|. |    .        . . | |   .     .|. || .| ..    ..         . | .  .|. .||||. || .  . 
WCVIKHIGYGGYGCVHRVIFVNNGTFRIDDDMYCMKTEQHFMQKHDQRLHIERNVMKFICKCEYQCKYWRHFCEVHDFHQHRDWWWIVMTMHGPSMEECC

ELMNN-NFNQD------TIIRVCIRTLLAIKTMHEYTYIHRDLKPCNFAVDFNP--NSLHVYLFDYGMARK-YATKDSGNKWQLRRPRNPAQFRGTVRYC
..| |  |         ..|.    .| .|  ||.  ..||..|| |.  . ..     . |  |||  |.      ..   |.| ||  . |||. .||
MMMKNFQFTLNEKKNQLCAIQCGYQCLNVIECMHWHGFHHRHMKPNNYCYMAPSQQHQHCYYMLDYGICRRVFFVSNNH---QIRQPRPITPFRGCKDYC

SLNMHKRKELSRVDDLWSWFFMLM
.   |. .|..| ||. |||.||.
PCSCHQCREHGRRDDMESWFYMLY
Kinase W09C3.1.AA Dual 93-284 28 3e-21 68.1 In-house 88-282 (282) Show / Hide 
Range on Protein: 93-284
Range on HMM: 88-282/282
Sequence Identity: 28% (57 aa)

YIVMTLCGPDLQKIAE-LMNNNFNQDTIIRVCIRTLLAIKTMHEYTYIHRDLKPCNFAVDFNPNSLHVYLFDYGMARKYATKDSGNKWQLRRPRNPAQFR
|.|| . || || |    ... .. ..   | | .| ||.  |   |.||. || ||..  .     ||. |....|||   |  || . | .|    . 
YWVMSPYGPSLQEIRRKYIMGQLSMHCSFNVAIQMLRAIQDLHSIGYLHRNIKPANFHCGLGMDETTVYMQDFRIVRKYI--DPENK-KHRTARKKVKMY

GTVRYCSLNMHKRKELSRVDDLWSWFFM---LMEMYAPLPWTSSNIPERIEALKEDHLNEYLSKDSFLSS--FLPLVEYLNSVQYADRPDYMKIYDIL
|. || .    . |.  | ||| .|  |   ||     ..| ... .  .   |.      ....    .  . ||| | |. ||   |||  .   |
GCGRYACRACMMQKDQGRKDDLECWIYMVFDLMDPEHGMSWRKECDCNMMIKQKDNFMYHFDKHQYSHIPRRLKPLVSYVNGYQYDSTPDYDYFRNCL
Kinase W09C3.1.AA Pkinase 23-198 24 2.5e-17 62.36 Pfam 1-179 (293) Show / Hide 
Range on Protein: 23-198
Range on HMM: 1-179/293
Sequence Identity: 24% (48 aa)

WLTIRELGRGAFGTVYHVSNKSTKQEAALKIEKMTAGDNLLKF-------EREIMVAMQHEP--TAIHIYDDGIYKDYRYIVMTLCGPDLQKIAELM---
. ..| .|.|.||.||.  .|.| .. |.|| |   ...  |        | .||  ..|.   . .... |   ||. |.|| .|. .|   ..|.   
YHIGRKIGSGSFGCVYKCHHKGTGKIVAVKIIKKR-HEKSWKDQTHRRLSEIQIMKRLNHPNIVRLYDWFED---KDHIYMVMEYCEGGLY-VMDLFDYI

----NNNF--NQDTIIRVCIRTLLAIKTMHEYTYIHRDLKPCNFAVDFNPNSL--HVYLFDYGMARKYATKDSGNKWQLRRPRNPAQFRGTVRYCS
    ...|    .......  .| ... .|.   ||||||| |. .| | . .  .| . | | |...          . . . .  | || .|..
STWRHGCFYFSEWECKFYMYQILRGLEYCHSMGIIHRDLKPENILID-NNGHIDACVKICDFGLAKQFD---------Y-NNS-MTTFCGTPWYMA
Kinase W09C3.1.AA S_TKc 23-286 20 2.7e-15 47.36 SMART 1-231 (231) Show / Hide 
Range on Protein: 23-286
Range on HMM: 1-231/231
Sequence Identity: 20% (57 aa)

WLTIRELGRGAFGTVYHVSNKSTKQEAALKIEKMTAGDNLLKFEREIMVAMQHEPTAIHIYDDGIYKDYRYIVMTLCGP---DLQKIAELMNNN-----F
.  .|..|.||||.|| . .|.|... |.|| |        ...||| . ..| |  ...||     |. |.|| .|.    || .. . .. .     |
YEILRKIGKGAFGKVYKCRHKKTGRIVAIKIIK-------EHIRREIQILKKHHPNIVKLYDVFQD-DHLYMVMEYCDGDLGDLFDYIKKRGRHGLRFPF

NQDTIIRVCIRTLLAIKTMHEYTYIHRDLKPCNFAVDFNPNSLHVYLFDYGMARKYATKDSGNKWQLRRPRNPAQFRGTVRYCSLNMHKRKELSR-VDDL
.  .. ...  ...|.. .|.   ||||||| |  .|      |. . | |.||..                   | ||  |..  .      ..   | 
PE-HARFYMYQICCALEYCHSHGIIHRDLKPENILLDE-----HIKICDFGLARQL-----------------TTFCGTPWYMAPEVL---GYGKCKCDW

WSW---FFMLMEMYAPLPWTSSNIPERIEALKEDHLNEYLSKDSFLSSFLPLVEYLNSVQYADRPDYMKIYD-------ILYA
||    .. ..  | | |    .. . .. ..              .... ... .  .  ..||   ..           . 
WSCGCILYEMLCGYPPFP----QMQMMFKKIG-------------SPEAKDFIRKCLQKDPEKRPTA-EALQDEWDIKCHPWF
Kinase W09C3.1.AA TyrKc 23-284 18 9.5e-05 -145.88 SMART 1-280 (280) Show / Hide 
Range on Protein: 23-284
Range on HMM: 1-280/280
Sequence Identity: 18% (57 aa)

WLTIRELGRGAFGTVYHVSNKSTKQEAALKIEKMTAGDNLLKFEREIMVAMQHE----PTAIHIYDDGIYK-------DYRYIVMTLCGP-DLQKIAELM
   ...||.||||.||.   |   .. | |  |  |   .  | ||. . | .     |  . .|   . .       .  .||| ..   ||..     
ITLGKKLGEGAFGEVYKGTWK---IQVAVKMLKEDA---REDFMREARI-MCKLGGHHPNIVRLYGVCTQQGRNFMYIEPLMIVMEYMPHGDLKDYLRKH

NNNFNQDTIIRVCIRTLLAIKT--------MHEYTYIHRDLKPCNFAVDFNPNSLHVYLFDYGMARKYATK-DSGNKWQLRRPRNPA----QFR--GTVR
 ..        .| ..|| . .         |.  .|||||   |  |. |    .|   | |..|.   .  .|     .     .    . .    .|
RPK--------LCMKDLLSFCWQIACGMEYLHSKNCIHRDLAARNCLVGENH---TVKISDFGLSRDIYDDDQQG----ESKDYYRKKEHKGGKTMLPIR

YCSLNMHKRKELSRVDDLWSWFFMLMEM--YAPLPWTS-----SNIPERIEALKEDHLNEYLSKD-------SFLSSFLPLVEYLNSVQYADRPDYMKIY
 ...   . .. .   | ||.  .. |.  |   |.        .  | || .|. .  .    |            .  . .     . .|||    |.
WMAPECINYGKFTTKSDVWSFGVCMWEIFTYGQQPYPGMIQQMMSNQEVIEYVKQGYRMPQPPNDYPMSFVCCPDEMYQ-IMKQCWHEDPEDRPTFSEIH

DIL
...
ECF
Kinase W09C3.1.AA Dual 23-48 38 0.000945 8.34 In-house 1-26 (282) Show / Hide 
Range on Protein: 23-48
Range on HMM: 1-26/282
Sequence Identity: 38% (10 aa)

WLTIRELGRGAFGTVYHVSNKSTKQE
|  .. || | |. ||||   | . .
WKVVNLLGSGGFRDVYHVHRFSQVNP
Kinase W09C3.1.AA CK1 264-278 33 0.133765 1.52 In-house 257-271 (271) Show / Hide 
Range on Protein: 264-278
Range on HMM: 257-271/271
Sequence Identity: 33% (5 aa)

EYLNSVQYADRPDYM
.| .. .....||||
NYCRQLHFEEKPDYM