Difference between revisions of "Kinase Family DYRK"
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DYRK ("'''D'''ual specificity T'''y'''rosine '''R'''egulated '''K'''inase") kinases have an unusual autophosphorylation on tyrosine, but otherwise phosphorylate only on Ser/Thr. The autophosphorylation occurs while still attached to the ribosome, and is an intramolecular reaction <cite>Lochhead1</cite>. Autophosphorylation occurs on a Y (YxY in DYRK1) in the [[activation loop]] which is almost absolutely conserved across all DYRK subfamilies. However, human Dyrk1A is active without this tyrosine phosphorylation, as shown in vitro in phosphatase-treated enzyme, or in mutants in which the substrate Ys are removed <cite>Adayev</cite>. | DYRK ("'''D'''ual specificity T'''y'''rosine '''R'''egulated '''K'''inase") kinases have an unusual autophosphorylation on tyrosine, but otherwise phosphorylate only on Ser/Thr. The autophosphorylation occurs while still attached to the ribosome, and is an intramolecular reaction <cite>Lochhead1</cite>. Autophosphorylation occurs on a Y (YxY in DYRK1) in the [[activation loop]] which is almost absolutely conserved across all DYRK subfamilies. However, human Dyrk1A is active without this tyrosine phosphorylation, as shown in vitro in phosphatase-treated enzyme, or in mutants in which the substrate Ys are removed <cite>Adayev</cite>. | ||
| − | DYRK | + | The DYRK family has the following subfamilies: |
| − | [[Kinase_Subfamily_DYRK1|DYRK1]] | + | ====[[Kinase_Subfamily_DYRK1|DYRK1]]==== |
| − | [[Kinase_Subfamily_DYRK2|DYRK2]] | + | ====[[Kinase_Subfamily_DYRK2|DYRK2]]==== |
| − | [[Kinase_Subfamily_PRP4|PRP4]] | + | ====[[Kinase_Subfamily_PRP4|PRP4]]==== |
| − | [[Kinase_Subfamily_HIPK|HIPK]] | + | ====[[Kinase_Subfamily_HIPK|HIPK]]==== |
Homeodomain-Interacting Protein Kinase. | Homeodomain-Interacting Protein Kinase. | ||
| − | [[Kinase_Subfamily_YAK|YAK]] | + | ====[[Kinase_Subfamily_YAK|YAK]]==== |
| − | [[Kinase_Subfamily_DYRKP|DYRKP]] | + | ====[[Kinase_Subfamily_DYRKP|DYRKP]]==== |
Originally plant-specific (hence the P) but now found in other lineages. | Originally plant-specific (hence the P) but now found in other lineages. | ||
Latest revision as of 03:49, 10 January 2022
Kinase Classification: Group CMGC: Family DYRK
DYRK ("Dual specificity Tyrosine Regulated Kinase") kinases have an unusual autophosphorylation on tyrosine, but otherwise phosphorylate only on Ser/Thr. The autophosphorylation occurs while still attached to the ribosome, and is an intramolecular reaction [1]. Autophosphorylation occurs on a Y (YxY in DYRK1) in the activation loop which is almost absolutely conserved across all DYRK subfamilies. However, human Dyrk1A is active without this tyrosine phosphorylation, as shown in vitro in phosphatase-treated enzyme, or in mutants in which the substrate Ys are removed [2].
The DYRK family has the following subfamilies:
DYRK1
DYRK2
PRP4
HIPK
Homeodomain-Interacting Protein Kinase.
YAK
DYRKP
Originally plant-specific (hence the P) but now found in other lineages.
References
- Lochhead PA, Sibbet G, Morrice N, and Cleghon V. Activation-loop autophosphorylation is mediated by a novel transitional intermediate form of DYRKs. Cell. 2005 Jun 17;121(6):925-36. DOI:10.1016/j.cell.2005.03.034 |
- Adayev T, Chen-Hwang MC, Murakami N, Lee E, Bolton DC, and Hwang YW. Dual-specificity tyrosine phosphorylation-regulated kinase 1A does not require tyrosine phosphorylation for activity in vitro. Biochemistry. 2007 Jun 26;46(25):7614-24. DOI:10.1021/bi700251n |
