Difference between revisions of "Kinase Group NDK"

Revision as of 23:16, 21 February 2012

Kinase Classification: Group NDK

Nucleoside Diphosphate Kinases use ATP to transfer a phosphate to other nucleoside diphosphates, using a phospho-histidine intermediate. One of 9 human NDKs was shown to have protein kinase activity, and kinase activity was also reported for the Drosophila NDK, awd

This family is universal in eukaryotes, and best known to phosphorylate nucleoside diphosphates to nucleoside triphosphates. They have a ferridoxin-like fold, and use a phospho-histidine intermediate during catalysis (the donor phosphate is transiently attached to a His residue on the NDPK). There are 9 NDPK homologs in human, of which four (NME1-4) are orthologous to Drosophila awd. NME2 (NDPK-B) was shown to phosphorylate the KCa3.1 potassium channel calcium channel on a conserved histidine [1] and a G protein β subunit [2]. Several other mammalian proteins are reported to be histidine phosphorylated, including histone H4, Gβ1, annexin-1, P-selectin, and ornithine transcarbamylase, some of which may be substrates for NDPKs (no other human histidine kinases are known).

Serine kinase activity was also reported for NME2 [3], though the transfer appeared to be stoichiometric (i.e. no turnover: one enzyme, one phosphorylation action) and so termed a phosphotransferase. The Drosophila homolog, awd, was also seen to autophosphorylate and transphosphorylate (ovalbumin) on Serine [4] and an E. coli NDPK was also shown to autophosphorylate on both serine and histidine [5].

References

1. Srivastava S, Li Z, Ko K, Choudhury P, Albaqumi M, Johnson AK, Yan Y, Backer JM, Unutmaz D, Coetzee WA, and Skolnik EY. Histidine phosphorylation of the potassium channel KCa3.1 by nucleoside diphosphate kinase B is required for activation of KCa3.1 and CD4 T cells. Mol Cell. 2006 Dec 8;24(5):665-675. DOI:10.1016/j.molcel.2006.11.012 | PubMed ID:17157250 | HubMed [Srivastava]
2. Hippe HJ, Lutz S, Cuello F, Knorr K, Vogt A, Jakobs KH, Wieland T, and Niroomand F. Activation of heterotrimeric G proteins by a high energy phosphate transfer via nucleoside diphosphate kinase (NDPK) B and Gbeta subunits. Specific activation of Gsalpha by an NDPK B.Gbetagamma complex in H10 cells. J Biol Chem. 2003 Feb 28;278(9):7227-33. DOI:10.1074/jbc.M210305200 | PubMed ID:12486122 | HubMed [Cuello]
4. Inoue H, Takahashi M, Oomori A, Sekiguchi M, and Yoshioka T. A novel function for nucleoside diphosphate kinase in Drosophila. Biochem Biophys Res Commun. 1996 Jan 26;218(3):887-92. DOI:10.1006/bbrc.1996.0158 | PubMed ID:8579610 | HubMed [Inoue]
5. Almaula N, Lu Q, Delgado J, Belkin S, and Inouye M. Nucleoside diphosphate kinase from Escherichia coli. J Bacteriol. 1995 May;177(9):2524-9. DOI:10.1128/jb.177.9.2524-2529.1995 | PubMed ID:7730286 | HubMed [Almaula]

Unlinked References

1. Engel pmid=8529641