Kinase Group NDK
Kinase Classification: Group NDK
Nucleoside Diphosphate Kinases use ATP to transfer a phosphate to other nucleoside diphosphates, using a phospho-histidine intermediate. One of 9 human NDKs was shown to have protein kinase activity, and kinase activity was also reported for the Drosophila NDK, awd
This family is universal in eukaryotes, and best known to phosphorylate nucleoside diphosphates to nucleoside triphosphates. They have a ferridoxin-like fold, and use a phospho-histidine intermediate during catalysis (the donor phosphate is transiently attached to a His residue on the NDPK). There are 9 NDPK homologs in human, of which four (NME1-4) are orthologous to Drosophila awd. NME2 (NDPK-B) was shown to phosphorylate the KCa3.1 potassium channel calcium channel on a conserved histidine [1] and a G protein β subunit [2]. Several other mammalian proteins are reported to be histidine phosphorylated, including histone H4, Gβ1, annexin-1, P-selectin, and ornithine transcarbamylase, some of which may be substrates for NDPKs (no other human histidine kinases are known).
Serine kinase activity was also reported for NME2 [3], though the transfer appeared to be stoichiometric (i.e. no turnover: one enzyme, one phosphorylation action) and so termed a phosphotransferase. The Drosophila homolog, awd, was also seen to autophosphorylate and transphosphorylate (ovalbumin) on Serine [4] and an E. coli NDPK was also shown to autophosphorylate on both serine and histidine [5].
References
- Inoue H, Takahashi M, Oomori A, Sekiguchi M, and Yoshioka T. A novel function for nucleoside diphosphate kinase in Drosophila. Biochem Biophys Res Commun. 1996 Jan 26;218(3):887-92. DOI:10.1006/bbrc.1996.0158 |
- Almaula N, Lu Q, Delgado J, Belkin S, and Inouye M. Nucleoside diphosphate kinase from Escherichia coli. J Bacteriol. 1995 May;177(9):2524-9. DOI:10.1128/jb.177.9.2524-2529.1995 |
Unlinked References
- Engel pmid=8529641
