TKL-Sp1 Draft Analysis

Kinase Classification: Group TKL: Family TKL-Sp1: TKL-Sp1 Draft Analysis

Draft Analysis of TKL-Sp1

This data is preliminary, incomplete, and may even be incorrect in areas. It is presented here to share early unpublished results that might otherwise languish. Please use with caution and credit the Manning lab if you wish to publish this data or conclusions from it.

Sequences and Phylogeny

Vertebrate Members

TKL-Sp1 is found in all fish and one amphibian. One member from Danio is published as 'CBL-interacting protein kinase 26-like' (XP_002665081.2). It, and a Medaka homolog are fully covered by ESTs apart from the C-terminal end. Genomic predictions show homologs in Stickleback (2 copies), Fugu and Tetraodon. A single EST from the amphibian axolotl (gb|CO779758.1) is also clearly orthologous, but not other evidence of tetrapod homologs could be found (Aug 2011).

The conserved start of the fish members is supported by some upstream EST coverage in Danio which is not conserved and the presence of an upstream stop in a trout EST.

Other Deuterostomes

Independent expansions of the TKL-Sp1 family are seen in the hemichordate Saccoglossus, with 4 members (two very similar pairs) and the sea urchin (echinoderm) Strongylocentrotus purpuratus, with 10 members. Many of the urchin members have putative upstream regions, some composed of ankyrin repeats (common in many urchin kinases), though these sequences have no EST coverage. A single homolog is seen in the chordate Branchiostoma, and none is seen in either Ciona genome.

Other Animals

Searches of genome predictions, NRAA and EST databases show two orphan members of TKL-Sp1, in widely divergent species: the wasp, Nasonia vitripennis, and the Cnidarian, Nematostella vectensis. These have no homologs in other insects or other cnidarians, respectively.

Sequences and Alignments

An initial alignment of the above sequences is found at tkl-sp1.aln.

Gerard 07:10, 4 August 2011 (PDT)