TKL-Sp1 Draft Analysis

Kinase Classification: Group TKL: Family TKL-Sp1: TKL-Sp1 Draft Analysis

Draft Analysis of TKL-Sp1

This data is preliminary, incomplete, and may even be incorrect in areas. It is presented here to share early unpublished results that might otherwise languish. Please use with caution and credit the Manning lab if you wish to publish this data or conclusions from it.

Sequences and Phylogeny

Vertebrate Members

One member from Danio is published as 'CBL-interacting protein kinase 26-like' (XP_002665081.2). Comparative gene finding shows clearly orthologous genes in other fish: Fugu, Tetraodon, Stickleback (2 copies) and Medaka and. A single EST from the amphibian axolotl (gb|CO779758.1) is also clearly orthologous, but not other evidence of tetrapod homologs could be found (Aug 2011). . The conserved start of the fish members is supported by full EST coverage of the Danio sequence, lack of sequence conservation in the 5' UTR region of Danio, and the presence of an upstream stop in a trout EST. The Medaka sequence is also full covered by ESTs, while the other fish are homology-based predictions.

Other Deuterostomes

Independent expansions of the TKL-Sp1 family are seen in the hemichordate Saccoglossus, with 4 members (two very similar pairs) and the sea urchin (echinoderm) Strongylocentrotus purpuratus, with 10 members. Many of the urchin members have putative upstream regions, some composed of ankyrin repeats (common in many urchin kinases), though these sequences have no EST coverage. A single homolog is seen in the chordate Branchiostoma, and none is seen in either Ciona genome.

Other Animals

Searches of genome predictions, NRAA and EST databases show two orphan members of TKL-Sp1, in widely divergent species: the wasp, Nasonia vitripennis, and the Cnidarian, Nematostella vectensis. These have no homologs in other insects or other cnidarians, respectively.