Kinase Family SgK071
SgK071 is holozoan kinase of unknown function. Vertebrate SgK071 genes are likely pseudokinases.
SgK071 is found in holozoans, though lost in many clades, including sponges, nematodes, insects and fish. It is generally single-copy, but three copies are found in the sea urchin.
SgK071 has an N-terminal kinase domain followed by an unannotated region typically of the same length. Mammalian SgK071 have lost the catalytic D166 residue, changing HRDxxxxN to HRNxxxxN. The N71 is further lost in Xenopus, and vertebrates also have an unusual DFG motif, DLS. Invertebrate homologs from Ciona to Nematostella have the full HRD motif, and a typical DFG motif.
Almost nothing is known about the function of this gene. Human SgK071 was found in an RNAi screen for tamoxifen resistance in breast cancer cell lines . Mouse SgK071 ESTs also derive from mammary glands but are predominantly from testis, and BioGPS also shows almost exclusive testis expression in mouse. Most human SgK071 ESTs come from testis or various areas of the brain. ProteinAtlas.Org reports expression in the nucleus, and in the cytoplasm of one cell line and many tissues. Tissue expression was high in cortical cells of the prostate and adrenal gland.
- Gonzalez-Malerva L, Park J, Zou L, Hu Y, Moradpour Z, Pearlberg J, Sawyer J, Stevens H, Harlow E, and LaBaer J. High-throughput ectopic expression screen for tamoxifen resistance identifies an atypical kinase that blocks autophagy. Proc Natl Acad Sci U S A. 2011 Feb 1;108(5):2058-63. DOI:10.1073/pnas.1018157108 |